Gene expression profiling during thymus ontogeny and its association with TCRV&bgr;8.1‐D&bgr;2.1 rearrangements of inbred mouse strains
详细信息 查看全文
- 作者：Espanhol ; Aline R. ; Macedo ; Clá ; udia ; Junta ; Cristina M. ; Cardoso ; Renato Sousa ; Victorero ; Geneviè ; ve ; Loriod ; Beatrice ; Nguyen ; Catherine ; Jordan ; Bertrand ; Passos ; Geraldo A.S.
- 关键词：TCRV8.1 ; V(D)J recombination ; gene expression profiling ; cytokine ; cluster analysis
- 刊名：Molecular and Cellular Biochemistry
- 出版年：2003
- 出版时间：2003
- 年：2003
- 卷：252
- 期：1/2
- 页码：223-228
- 全文大小：81 KB
文摘
The V(D)J recombination of TCR&agr; and &bgr; in early developing T‐cells is a highly modulated phenomenon initiated and completed by recombinase complex (RAG‐1 and RAG‐2), and regulated by other gene products such as interleukins. To further evaluate the association of several other gene products with the evolution of TCRV&bgr;8.1 V(D)J rearrangements in vivo , the mRNA expression levels of seven interleukins, three cytokines, receptors TCRV&bgr;8.1 and IL‐2R&bgr;, MHC‐I/MHC‐II, RAG‐1/RAG‐2 and retroviral superantigen MMTV(SW) were measured by RT‐PCR during the fetal development of the thymus of three inbred mouse strains (Balb‐c, C57Bl/6 and CBA/J). Clustering using the Tree View software, was used to organize these genes based on similarity of expression patterns. Each strain displayed a different expression profile during thymus ontogeny.
During the late developmental stage the most evident association was the kinetics of MMTV(SW) retrovirus, IL‐2R&bgr; and IL‐7 overexpression with reduction of TCRV&bgr;8.1‐D&bgr;2.1 rearrangement in the thymus of CBA/J mice. These data suggest a susceptibility of this strain to expression of MMTV(SW) upon reduction of the rearranged TCRV&bgr;8.1‐D&bgr;2.1 segment in developing thymocytes, with parallel IL‐7 overexpression.