Genetic mapping of sequence-specificPCR-based markers on the short arm of the1BL.1RS wheat-rye translocation

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• 作者：Nagy ; E.D. ; Eder ; C. ; Moln&aacute ; r L&aacute ; ng ; M. ; Lelley ; T.
• 关键词：1BL.1RS translocation ; genetic mapping ; homologous recombination ; secalin ; SSR ; STS marker
• 刊名：Euphytica
• 出版年：2003
• 出版时间：2003
• 年：2003
• 卷：132
• 期：3
• 页码：243-249
• 全文大小：174 KB
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Plant Physiology
  Plant Sciences
  Ecology
  
• 出版者：Springer Netherlands
• ISSN：1573-5060

文摘

Wheat cultivars carrying the 1BL.1RStranslocation were crossed with newlysynthesised octoploid triticale linesinvolving four rye genotypes having &ogr;-secalin banding patternsdifferent from each other and from that ofthe 1BL.1RS translocation. Homologousrecombination was expected between theshort arm of the 1R chromosomes of the ryegenotypes and the 1RS arm of the 1BL.1RSwheat/rye translocation. Sevensequence-specific PCR-based markers:Xiag95, RMS13, Bmac0213, GPI, Xpsr960, 5Sand SCM9, and &ogr;-secalinproteins were used to detect recombinationevents in the BC₁F₂ generation.Segregation analysis demonstrated that abarley SSR marker (Bmac0213) locus waspresent on the 1RS chromosome arm. Of 834plants tested in four differentBC₁F₂ populations, 246individuals were found to carry recombined1BL.1RS translocation chromosomes. Geneticlinkage analysis was performed on the eightmarkers in the four different mappingpopulations. The physical positions of themarkers are discussed.