Expression of ornithine decarboxylase of Coccidioides immitis in three Escherichia coli strains carrying the lambda DE3 lysogen and an E. coli EWH319 strain odc

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• 作者：Pantoja Hern&aacute ; ndez ; Miguel Angel ; Muñ ; oz S&aacute ; nchez ; Claudia Ivonne ; Guevara Gonz&aacute ; lez ; Ramó ; n Gerardo ; Botello Alvarez ; Enrique ; Gonz&aacute ; lez Chavira ; Mario Martí ; n ; Torres
• 关键词：Coccidioides immitis ; Escherichia coli ; gene function ; lambda DE3 lysogen ; protein expression
• 刊名：Biotechnology Letters
• 出版年：2004
• 出版时间：2004
• 年：2004
• 卷：26
• 期：1
• 页码：75-78
• 全文大小：99 KB
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Microbiology
  Biotechnology
  Applied Microbiology
  Biochemistry
  
• 出版者：Springer Netherlands
• ISSN：1573-6776

文摘

Ornithine decarboxylase from respiratory fungal pathogen, Coccidioides immitis, cloned in the pETCiODC plasmid under control of T7lac promoter, was produced in E. coli BL21(DE3), BL21(DE3)pLysS, BLR(DE3) and EWH319 transformant strains. E. coli BL21(DE3)pLysS-pETCiODC expressed the highest specific activity of ODC, suggesting that this strain could be successfully used for protein structure and drug testing studies.