Genetic Variability in Hoplias malabaricus (Osteichthyes: Erythrinidae) in Fluvial and Lacustrine Environments in the Upper Paraná River Floodplain (Paraná

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• 作者：Peres ; Maria Dolores ; Renesto ; Erasmo ; Lapenta ; Ana Sí ; lvia ; Zawadzki ; Cl&aacute ; udio Henrique
• 关键词：isozymes ; floodplain ; genetic variability ; Paran&aacute ; River ; Hoplias malabaricus ; fishes ; allozymes
• 刊名：Biochemical Genetics
• 出版年：2002
• 出版时间：2002
• 年：2002
• 卷：40
• 期：7/8
• 页码：209-223
• 全文大小：563 KB

文摘

Genetic variability in Hoplias malabaricus, from two localities in the upper Paraná River floodplain, was investigated by starch and polyacrylamide gel electrophoresis. A total of 52 specimens were analyzed for 14 enzymatic systems. Twenty-three gene loci of 13 enzymatic systems (AAT, ACP, ADH, GDH, G6PDH, GPI, IDH, LDH, MDH, MEP, PGM, PER, and SOD) were analyzed by starch gel electrophoresis (Penetrose-30). The EST system was analyzed by polyacrylamide gel electrophoresis, and one polymorphic locus was found (EST-1). Twenty-four loci were detected. The proportion of polymorphic loci was 37.5% in the lagoon and 33.3% in the river. Significant differences in allele frequencies of five loci were found between specimens from the two environments. Expected mean heterozygosity (H_e = 0.14) is the same in the river and lagoon, however, Nei's genetic distance (D) between the population of the two locations was 0.049.