Characterization of microbial communities in heavy crude oil from Saudi Arabia

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• 作者：Majed Albokari (1)
  Ibrahim Mashhour (1)
  Mohammed Alshehri (1)
  Chris Boothman (2)
  Mousa Al-Enezi (3)
  
  1. Atomic Energy Research Institute (AERI) ; King Abdulaziz City for Science and Technology (KACST) ; P. O. Box 6086 ; Riyadh ; 11442 ; Saudi Arabia
  2. School of Earth ; Atmospheric and Environmental Sciences and Williamson Research Centre for Molecular Environmental Science ; University of Manchester ; Manchester ; M13 9PL ; UK
  3. Saudi Aramco ; Research & Development Center ; P.O. Box 62 ; Dhahran ; 31311 ; Saudi Arabia
  
• 关键词：Heavy crude oil ; Oil sludge ; 16S rRNA ; PCR amplification ; Saudi Aramco oil company
• 刊名：Annals of Microbiology
• 出版年：2015
• 出版时间：March 2015
• 年：2015
• 卷：65
• 期：1
• 页码：95-104
• 全文大小：699 KB
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• 刊物主题：Microbiology; Microbial Genetics and Genomics; Microbial Ecology; Fungus Genetics; Medical Microbiology; Applied Microbiology;
• 出版者：Springer Berlin Heidelberg
• ISSN：1869-2044

文摘

The complete mineralization of crude oil into carbon dioxide, water, inorganic compounds and cellular constituents can be carried out as part of a bioremediation strategy. This involves the transformation of complex organic contaminants into simpler organic compounds by microbial communities, mainly bacteria. A crude oil sample and an oil sludge sample were obtained from Saudi ARAMCO Oil Company and investigated to identify the microbial communities present using PCR-based culture-independent techniques. In total, analysis of 177 clones yielded 30 distinct bacterial sequences. Clone library analysis of the oil sample was found to contain Bacillus, Clostridia and Gammaproteobacteria species while the sludge sample revealed the presence of members of the Alphaproteobacteria, Betaproteobacteria, Gammaproteobacteria, Clostridia, Spingobacteria and Flavobacteria. The dominant bacterial class identified in oil and sludge samples was found to be Bacilli and Flavobacteria, respectively. Phylogenetic analysis showed that the dominant bacterium in the oil sample has the closest sequence identity to Enterococcus aquimarinus and the dominant bacterium in the sludge sample is most closely related to the uncultured Bacteroidetes bacterium designated AH.KK.