Lectin of Abelmoschus esculentus (okra) promotes selective antitumor effects in human breast cancer cells

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• 作者：Leonardo G. Monte (1)
  Tatiane Santi-Gadelha (4)
  Larissa B. Reis (1)
  Elizandra Braganhol (5)
  Rafael F. Prietsch (5)
  Odir A. Dellagostin (2)
  Rodrigo Rodrigues e Lacerda (4)
  Carlos A. A. Gadelha (4)
  Fabricio R. Concei??o (3)
  Luciano S. Pinto (1)
  
• 关键词：Abelmoschus esculentus ; Apoptosis ; Cancer inhibition ; Human breast cancer ; Lectin ; Okra ; Plants
• 刊名：Biotechnology Letters
• 出版年：2014
• 出版时间：March 2014
• 年：2014
• 卷：36
• 期：3
• 页码：461-469
• 全文大小：405 KB
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• 作者单位：Leonardo G. Monte (1)
  Tatiane Santi-Gadelha (4)
  Larissa B. Reis (1)
  Elizandra Braganhol (5)
  Rafael F. Prietsch (5)
  Odir A. Dellagostin (2)
  Rodrigo Rodrigues e Lacerda (4)
  Carlos A. A. Gadelha (4)
  Fabricio R. Concei??o (3)
  Luciano S. Pinto (1)
  
  1. Laboratório de Biotecnologia Vegetal e Prote?mica, Núcleo de Biotecnologia, Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, P.O. Box 354, Pelotas, RS, 96010-900, Brazil
  4. Departamento de Biologia Molecular, Universidade Federal da Paraíba, Cidade Universitária, Jo?o Pessoa, PB, CEP 58059-900, Brazil
  5. Centro de Ciências Químicas, Farmacêuticas e de Alimentos, Universidade Federal de Pelotas, Pelotas, RS, 96010-900, Brazil
  2. Laboratório de Vacinologia, Núcleo de Biotecnologia, Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Pelotas, RS, 96010-900, Brazil
  3. Laboratório de Imunologia Aplicada, Núcleo de Biotecnologia, Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Pelotas, RS, 96010-900, Brazil
  
• ISSN：1573-6776

文摘

The anti-tumor effects of a newly-discovered lectin, isolated from okra, Abelmoschus esculentus (AEL), were investigated in human breast cancer (MCF7) and skin fibroblast (CCD-1059 sk) cells. AEL induced significant cell growth inhibition (63?%) in MCF7 cells. The expression of pro-apoptotic caspase-3, caspase-9, and p21 genes was increased in MCF7 cells treated with AEL, compared to those treated with controls. In addition, AEL treatment increased the Bax/Bcl-2 ratio in MCF7 cells. Flow cytometry also indicated that cell death (72?%) predominantly occurred through apoptosis. Thus, AEL in its native form promotes selective antitumor effects in human breast cancer cells and may represent a potential therapeutic to combat human breast cancer.