TNF-α, IL-6, and IL-8 Cytokines and Their Association with TNF-α-308 G/A Polymorphism and Postoperative Sepsis

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• 作者：Kavita Baghel (1)
  Rajeshwar Nath Srivastava (2)
  Abhijit Chandra (1)
  Sudhir K. Goel (3)
  Jyotsna Agrawal (4)
  Hasan Raza Kazmi (1)
  Saloni Raj (1)
  
• 关键词：Cytokines ; Genetic polymorphism ; Restriction fragment length polymorphism ; Sepsis ; Tumor necrosis factor
• 刊名：Journal of Gastrointestinal Surgery
• 出版年：2014
• 出版时间：August 2014
• 年：2014
• 卷：18
• 期：8
• 页码：1486-1494
• 全文大小：1,410 KB
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• 作者单位：Kavita Baghel (1)
  Rajeshwar Nath Srivastava (2)
  Abhijit Chandra (1)
  Sudhir K. Goel (3)
  Jyotsna Agrawal (4)
  Hasan Raza Kazmi (1)
  Saloni Raj (1)
  
  1. Department of Surgical Gastroenterology, King George’s Medical University, Lucknow, 226003, India
  2. Department of Physical Medicine and Rehabilitation, King George’s Medical University, Lucknow, 226003, India
  3. Department of Biochemistry, All India Institute of Medical Sciences, Bhopal, 462024, India
  4. Department of Microbiology, King George’s Medical University, Lucknow, 226003, India
  
• ISSN：1873-4626

文摘

Introduction Early prediction of postoperative sepsis remains an enormous clinical challenge. Association of TNF-α-308 G/A polymorphism with sepsis remains controversial. We, therefore, investigated this polymorphism with serum levels of cytokines TNF-α, IL-6, and IL-8 in relation to development of sepsis following major gastrointestinal surgery. Methods Two hundred and thirty-nine patients undergoing major gastrointestinal surgery were enrolled. Polymorphism was studied through the analysis of restriction fragments of Nco1-digested DNA with the polymerase chain reaction. All patients were followed for 1?month following surgery for evidence of sepsis. Levels of serum cytokines TNF-α, IL-6, and IL-8 were measured preoperatively and postoperatively by enzyme-linked immunosorbent assay (ELISA). Results Forty-seven (19.66?%) patients developed postoperative sepsis. Patients with postoperative sepsis were significantly (p--.002) more likely to possess AA homozygous genotype with higher capacity to produce cytokines TNF-α (p--.0001), IL-6 (p--.0001), and IL-8 (p--.0001) as compared to other genotypes. When compared with patients carrying at least one G allele, the AA genotype was associated with a significantly higher probability (odds ratio (OR)--.17; p--.003; 95?% confidence interval (CI)--.5-1.48) of developing sepsis. Compared with the GG genotype, AA was associated with a significantly higher probability (OR--.18; p--.0008; 95?% CI--.82-4.76) of sepsis development. Conclusion TNF-α-308 G/A polymorphism is significantly associated with the development of postoperative sepsis and with increased expression of cytokines TNF-α, IL-6, and IL-8.