Involvement of alanine racemase in germination of Bacillus cereus spores lacking an intact exosporium

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• 作者：Elena Venir (1)
  Manuela Del Torre (1)
  Vincenzo Cunsolo (2)
  Rosaria Saletti (2)
  Rita Musetti (3)
  Mara Lucia Stecchini (1)
  
• 关键词：Alanine racemase ; d ; Cycloserine ; Proteomics
• 刊名：Archives of Microbiology
• 出版年：2014
• 出版时间：February 2014
• 年：2014
• 卷：196
• 期：2
• 页码：79-85
• 全文大小：407 KB
• 作者单位：Elena Venir (1)
  Manuela Del Torre (1)
  Vincenzo Cunsolo (2)
  Rosaria Saletti (2)
  Rita Musetti (3)
  Mara Lucia Stecchini (1)
  
  1. Department of Food Science, University of Udine, Via Sondrio 2/A, 33100, Udine, Italy
  2. Department of Chemical Science, University of Catania, Viale A Doria 6, 95125, Catania, Italy
  3. Department of Biology and Plant Protection, University of Udine, Via delle Scienze 208, 33100, Udine, Italy
  
• ISSN：1432-072X

文摘

The l-alanine mediated germination of food isolated Bacillus cereus DSA 1 spores, which lacked an intact exosporium, increased in the presence of d-cycloserine (DCS), which is an alanine racemase (Alr) inhibitor, reflecting the activity of the Alr enzyme, capable of converting l-alanine to the germination inhibitor d-alanine. Proteomic analysis of the alkaline extracts of the spore proteins, which include exosporium and coat proteins, confirmed that Alr was present in the B. cereus DSA 1 spores and matched to that encoded by B. cereus ATCC 14579, whose spore germination was strongly affected by the block of conversion of l- to d-alanine. Unlike ATCC 14579 spores, l-alanine germination of B. cereus DSA 1 spores was not affected by the preincubation with DCS, suggesting a lack of restriction in the reactant accessibility.