Engineering Tobacco to Remove Mercury from Polluted Soil

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• 作者：S. Chang ; F. Wei ; Y. Yang ; A. Wang ; Z. Jin ; J. Li…
• 关键词：Phytoremediation ; Mercury ; Vacuole ; Cytoplasm ; Chelatin
• 刊名：Applied Biochemistry and Biotechnology
• 出版年：2015
• 出版时间：April 2015
• 年：2015
• 卷：175
• 期：8
• 页码：3813-3827
• 全文大小：3,554 KB
• 参考文献：1. Wu, GH, Cao, SS (2010) Mercury and cadmium contamination of irrigation water, sediment, soil and shallow groundwater in a waste water-irrigated field in Tianjin. China Bulletin Environmental Contamination and Toxicology 84: pp. 336-341 CrossRef
  2. Cachada, A, Rodrigues, SM, Mieiro, C, Ferreira Silva, E, Pereira, E, Duarte, AC (2009) Controlling factors and environmental implications of mercury contamination in urban and agricultural soils under a long-term influence of a chlor-alkali plant in the North–West Portugal. Environmental Geology 57: pp. 91-98 CrossRef
  3. Dunlevy, SR, Singleton, DR, Aitken, MD (2013) Biostimulation reveals functional redundancy of anthracene-degrading bacteria in polycyclic aromatic hydrocarbon-contaminated soil. Environmental Engineering Science 30: pp. 697-705 CrossRef
  4. Sorkhoh, NA, Ali, N, Al-Awadhi, H, Dashti, N, Al-Mailem, DM, Eliyas, M, Radwan, SS (2010) Phytoremediation of mercury in pristine and crude oil contaminated soils: contributions of rhizobacteria and their host plants to mercuryremoval. Ecotoxicology and Environmental Safety 73: pp. 1998-2003 CrossRef
  5. Francesca, P, Gianniantonio, P, Meri, BET, Paolo, A, Leonardo, P (2011) Mercury mobilization in a contaminated Industrial soil for phytoremediation. Communications in Soil Science and Plant Analysis 42: pp. 2767-2777 CrossRef
  6. Hussein, HS, Ruiz, ON, Terry, N, Daniell, H (2007) Phytoremediation of mercury and organomercurials in chloroplast transgenic plants: enhanced root uptake, translocation to shoots, and volatilization. Environmental Science & Technology 41: pp. 8439-8446 CrossRef
  7. Kiyono, M, Uno, Y, Omura, T, Pan-Hou, H (2000) Role of merT and merP from Pseudomona K-62 plasmid pmr26 in the transport of phenylmercury. Biological & Pharmaceutical Bulletin 23: pp. 279-282 CrossRef
  8. Kiyono, M, Omura, T, Fujimori, H, Pan-Hou, H (1995) Organomercurial resistance determinants in Pseudomonas K-62 are present on two plasmids. Archives of Microbiology 163: pp. 242-247 CrossRef
  9. Kiyono, M, Pan-Hou, H (2006) Genetic engineering of bacteria for environmental remediation of mercury. Journal of Health Science 52: pp. 199-204 CrossRef
  10. Osborn, AM, Bruce, KD, Strike, P, Ritchie, DA (1997) Distribution, diversity and evolution of the bacterial mercury resistance (mer) operon. FEMS Microbiology Reviews 19: pp. 239-262 CrossRef
  11. Ruiz, ON, Hussein, HS, Terry, N, Daniell, H (2003) Phytoremediation of organomercurial compounds via chloroplast genetic engineering. Plant Physiology 132: pp. 1344-1352 CrossRef
  12. Shafiul, H, Zeyaullah, M, Nabi, G, Srivastava, PS, Ali, A (2010) Transgenic tobacco plant expressing environmental E.coli merA gene for enhanced volatilization of ionic mercury. Journal of Micriobiology and Biotechnolology 20: pp. 917-924 CrossRef
  13. Nagata, T, Ishikawa, C, Kiyono, M, Pan-Hou, H (2006) Accumulation of mercury in transgenic tobacco expressing bacterial polyphosphate. Biological & Pharmaceutical Bulletin 29: pp. 2350-2353 CrossRef
  14. Hirata, K, Tsuji, N, Miyamoto, K (2005) Biosynthetic regulation of phytochelatins, heavy metal-binding peptides. Journal of Bioscience and Bioengineering 100: pp. 593-599 CrossRef
  15. Sun, H, Lang, Z, Zhu, L, Huang, D (2012) Acquiring transgenic tobacco
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Biotechnology
  Biochemistry
  
• 出版者：Humana Press Inc.
• ISSN：1559-0291

文摘

Tobacco is an ideal plant for modification to remove mercury from soil. Although several transgenic tobacco strains have been developed, they either release elemental mercury directly into the air or are only capable of accumulating small quantities of mercury. In this study, we constructed two transgenic tobacco lines: Ntk-7 (a tobacco plant transformed with merT-merP-merB1-merB2-ppk) and Ntp-36 (tobacco transformed with merT-merP-merB1-merB2-pcs1). The genes merT, merP, merB1, and merB2 were obtained from the well-known mercury-resistant bacterium Pseudomonas K-62. Ppk is a gene that encodes polyphosphate kinase, a key enzyme for synthesizing polyphosphate in Enterobacter aerogenes. Pcs1 is a tobacco gene that encodes phytochelatin synthase, which is the key enzyme for phytochelatin synthesis. The genes were linked with LP4/2A, a sequence that encodes a well-known linker peptide. The results demonstrate that all foreign genes can be abundantly expressed. The mercury resistance of Ntk-7 and Ntp-36 was much higher than that of the wild type whether tested with organic mercury or with mercuric ions. The transformed plants can accumulate significantly more mercury than the wild type, and Ntp-36 can accumulate more mercury from soil than Ntk-7. In mercury-polluted soil, the mercury content in Ntp-36’s root can reach up to 251?μg/g. This is the first report to indicate that engineered tobacco can not only accumulate mercury from soil but also retain this mercury within the plant. Ntp-36 has good prospects for application in bioremediation for mercury pollution.