Amplification and sequence analysis of phaC gene of polyhydroxybutyrate producing Vibrio azureus BTKB33 isolated from marine sediments

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• 作者：Raghul Subin Sasidharan ; Sarita Ganapathy Bhat…
• 关键词：Polyhydroxyalkanoates ; Vibrio azureus ; Molecular characterization ; Phylogenetic tree ; PHA synthase gene
• 刊名：Annals of Microbiology
• 出版年：2016
• 出版时间：March 2016
• 年：2016
• 卷：66
• 期：1
• 页码：299-306
• 全文大小：1,454 KB
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• 作者单位：Raghul Subin Sasidharan (1)
  Sarita Ganapathy Bhat (1)
  Muthusamy Chandrasekaran (1) (2)
  
  1. Department of Biotechnology, Cochin University of Science and Technology, Kalamassery, Cochin-22, Kerala, India
  2. Department of Botany & Microbiology, College of Science, King Saud University, PB No. 2455, Riyadh, 11451, Kingdom of Saudi Arabia
  
• 刊物主题：Microbiology; Microbial Genetics and Genomics; Microbial Ecology; Fungus Genetics; Medical Microbiology; Applied Microbiology;
• 出版者：Springer Berlin Heidelberg
• ISSN：1869-2044

文摘

Vibrios have shown their potential as polyhydroxyalkanoates (PHAs) producers, but until recently little information was available about their PHA-related genes. The present study attempts to characterize the phaC genes from a potent PHA-accumulating bacterium, Vibrio azureus strain BTKB33, isolated from marine sediments. The molecular detection of class I PHA synthase gene in the V. azureus strain BTKB33 gave the required amplicon and was confirmed by subsequent seminested PCR; however, a class II PHA synthase gene was not detected. The in silico characterization of the PCR product helped to deduce the presence of class I PHA synthase, particularly a polyhydroxybutyrate polymerase. Sequence alignment of the nucleotide sequence of class I PHA synthase of strain BTKB33 and other related Vibrio sp. showed intra-generic variation within genus Vibrio and this is revealed in the dendrogram. The multiple sequence alignment of the in silico-translated phaC gene of BTKB33 with the protein sequence of PHA synthase of related organisms showed the conserved regions of protein sequences within the genus Vibrio and the dendrogram constructed showed the relatedness based on the deduced amino acid sequences. Keywords Polyhydroxyalkanoates Vibrio azureus Molecular characterization Phylogenetic tree PHA synthase gene