Effects of Selenium on Morphological Changes in Candida utilis ATCC 9950 Yeast Cells

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• 作者：Marek Kieliszek ; Stanisław Błażejak…
• 关键词：Selenium ; Morphology ; Candida utilis ; Yeast cells
• 刊名：Biological Trace Element Research
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：169
• 期：2
• 页码：387-393
• 全文大小：1,033 KB
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• 作者单位：Marek Kieliszek (1)
  Stanisław Błażejak (1)
  Anna Bzducha-Wróbel (1)
  Agnieszka Kurcz (1)
  
  1. Department of Biotechnology, Microbiology and Food Evaluation, Faculty of Food Sciences, Warsaw University of Life Sciences—SGGW, Nowoursynowska 159 C, 02-776, Warsaw, Poland
  
• 刊物主题：Biochemistry, general; Biotechnology; Nutrition; Oncology;
• 出版者：Springer US
• ISSN：1559-0720

文摘

This paper presents the results of microscopic examinations of the yeast cells cultured in yeast extract–peptone–dextrose (YPD) media supplemented with sodium selenite(IV). The analysis of the morphological changes in yeast cells aimed to determine whether the selected selenium doses and culturing time may affect this element accumulation in yeast cell structures in a form of inorganic or organic compounds, as a result of detoxification processes. The range of characteristic morphological changes in yeasts cultivated in experimental media with sodium selenite(IV) was observed, including cell shrinkage and cytoplasm thickening of the changes within vacuole structure. The processes of vacuole disintegration were observed in aging yeast cells in culturing medium, which may indicate the presence of so-called ghost cells lacking intracellular organelles The changes occurring in the morphology of yeasts cultured in media supplemented with sodium selenite were typical for stationary phase of yeast growth. From detailed microscopic observations, larger surface area of the cell (6.03 μm2) and yeast vacuole (2.17 μm2) were noticed after 24-h culturing in the medium with selenium of 20 mg Se4+/L. The coefficient of shape of the yeast cells cultured in media enriched with sodium selenite as well as in the control YPD medium ranged from 1.02 to 1.22. Elongation of cultivation time (up to 48 and 72 h) in the media supplemented with sodium selenite caused a reduction in the surface area of the yeast cell and vacuole due to detoxification processes. Keywords Selenium Morphology Candida utilis Yeast cells