Optimization of Lactobacillus acidophilus cultivation using taro waste and evaluation of its biological activity
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- 作者：Shu-Chen Hsieh ; Jui-Ming Liu ; Xiao-Hui Pua…
- 关键词：Taro waste ; Lactobacillus acidophilus ; Heat ; killed cell ; Exopolysaccharide ; Immune regulation ; Anti ; tumor
- 刊名：Applied Microbiology and Biotechnology
- 出版年：2016
- 出版时间：March 2016
- 年：2016
- 卷：100
- 期：6
- 页码：2629-2639
- 全文大小：1,362 KB
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Jui-Ming Liu (2)
Xiao-Hui Pua (1)
Yuwen Ting (1)
Ren-Jun Hsu (3) (4)
Kuan-Chen Cheng (1) (5)
1. Graduate Institute of Food Science Technology, National Taiwan University, Taipei, 10617, Taiwan
2. Division of Urology, Department of Surgery, Taoyuan General Hospital, Ministry of Health and Welfare, 1492 Chung-Shan Road, Taoyuan District, Taoyuan, 330, Taiwan
3. Biobank Management Center of Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan
4. Department of Pathology and Graduate Institute of Pathology and Parasitology, Tri-Service General Hospital, National Defense Medical Center, No. 161, Sec. 6, Minquan E. Road, Neihu District, Taipei, 114, Taiwan
5. Institute of Biotechnology, National Taiwan University, No. 1, Sec. 4, Roosevelt Rd, Taipei, 10617, Taiwan - 刊物类别：Chemistry and Materials Science
- 刊物主题：Chemistry
Biotechnology
Microbiology
Microbial Genetics and Genomics - 出版者：Springer Berlin / Heidelberg
- ISSN：1432-0614
文摘
In this study, taro waste (TW) was utilized for Lactobacillus acidophilus BCRC 14079 cultivation and the anti-tumor and immune-modulatory properties of heat-killed cells (HKCs), cytoplasmic fraction (CF), and exopolysaccharide (EPS) were evaluated. The optimum liquefaction enzyme dosage, temperature, and time determined by Box-Behnken design response surface methodology (BBD-RSM) were 9 mL/L of α-amylase, 79.2 °C, and 5 h of reaction, respectively. The optimum temperature and reaction time for saccharification were determined as 60 °C and 3 h. The optimum medium, CGMY1 medium, constitutes of TW hydrolysate containing 37 g/L of glucose, 25 g/L of corn gluten meal (CGM), and 1 g/L of yeast extract (YE). Results of MTT assay showed that HKCs and EPS from CGM medium exhibited the highest anti-proliferative in HT-29 (IC50 of HKCs, 467.25 μg/mL; EPS, 716.10 μg/mL) and in Caco-2 cells (IC50 of EPS, 741.60 μg/mL). Luciferase-based NF-ΚB and COX-2 systems indicated HKCs from CGM medium stimulated the highest expression of luciferin in both systems. The luciferase activities by using 100 and 500 μg/mL of HKCs from CGM were 24.30- and 45.83-fold in NF-ΚB system and 11.54- and 4.93-fold in COX-2 system higher than the control. In conclusion, this study demonstrated the potential of TW medium for L. acidophilus cultivation and the production of non-viable probiotics with enhanced biological activities.