Joining the in vitro immunization of alpaca lymphocytes and phage display: rapid and cost effective pipeline for sdAb synthesis
详细信息 查看全文
- 作者：Lubos Comor ; Saskia Dolinska ; Katarina Bhide ; Lucia Pulzova…
- 关键词：VHH ; In vitro immunization ; OspA ; Phage display ; Single domain antibodies
- 刊名：Microbial Cell Factories
- 出版年：2017
- 出版时间：December 2017
- 年：2017
- 卷：16
- 期：1
- 全文大小：2319KB
- 刊物类别：Chemistry and Materials Science
- 刊物主题：Applied Microbiology; Biotechnology; Microbiology; Microbial Genetics and Genomics; Enzymology; Genetic Engineering;
- 出版者：BioMed Central
- ISSN：1475-2859
- 卷排序：16
文摘
BackgroundCamelids possess unique functional heavy chain antibodies, which can be produced and modified in vitro as a single domain antibody (sdAb or nanobody) with full antigen binding ability. Production of sdAb in conventional manner requires active immunization of Camelidae animal, which is laborious, time consuming, costly and in many cases not feasible (e.g. in case of highly toxic or infectious antigens).