Modulated expression of genes associated with NO signal transduction contributes to the cholesterol-lowering effect of electro-acupuncture

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• 作者：Ling Li (123) ling.li1980@gmail.com
  Guang-Hong Tan (2)
  Yi-Zheng Zhang (1)
• 关键词：Acupuncture &#8211 ; Electro ; acupuncture &#8211 ; Hypercholesterolemia &#8211 ; Suppression subtractive hybridization &#8211 ; nNOS &#8211 ; Mt1 &#8211 ; NO signal transduction
• 刊名：Biotechnology Letters
• 出版年：2012
• 出版时间：July 2012
• 年：2012
• 卷：34
• 期：7
• 页码：1175-1182
• 全文大小：262.4 KB
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• 作者单位：1. Center for Functional Genomics and Bioinformatics, College of Life Science, Sichuan University, Chengdu, 610064 Sichuan, China2. Hainan Provincial Key Laboratory of Tropical Medicine, Hainan Medical College, Haikou, 571101 Hainan, China3. West China Women鈥檚 and Children鈥檚 Hospital, Sichuan University, Chengdu, 610041 Sichuan, China
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Microbiology
  Biotechnology
  Applied Microbiology
  Biochemistry
  
• 出版者：Springer Netherlands
• ISSN：1573-6776

文摘

Electro-acupuncture (EA) at Fenglong acupoint (ST40) can lower the levels of serum cholesterol and triacylglycerols. To study the hepatic genes responsible for the cholesterol-lowering effect of EA, suppression subtractive hybridization combined with the switch mechanism at the 5′-end of RNA template cDNA synthesis and long-distance PCR were employed using hepatic tissues from hypercholesterolemia and EA-treated mice. 68 % of the identified genes are involved in metabolism, immune response, and signal transduction pathways. Real-time PCR and western blot indicate that EA at ST40 induces the expression of nNOS and Mt1, two genes involved in NO signal transduction. EA treatment for hypercholesterolemia thus involves the modulation of several biological pathways and provides a physiological link between NO signal transduction and the cholesterol-lowering effect of EA.