Palm tocotrienol-rich fraction inhibits methionine-induced cystathionine β-synthase in rat liver

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• 作者：Yusof Kamisah ; Ku-Zaifah Norsidah ; Ayob Azizi…
• 关键词：Tocotrienol ; Methionine ; Homocysteine ; S ; Adenosyl methionine ; S ; Adenosyl homocysteine ; Cystathionine β ; synthase
• 刊名：Journal of Physiology and Biochemistry
• 出版年：2015
• 出版时间：December 2015
• 年：2015
• 卷：71
• 期：4
• 页码：659-667
• 全文大小：433 KB
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• 作者单位：Yusof Kamisah (1)
  Ku-Zaifah Norsidah (1) (2)
  Ayob Azizi (3)
  Othman Faizah (4)
  Mohd Rizal Nonan (5)
  Ahmad Yusof Asmadi (6)
  
  1. Department of Pharmacology, Faculty of Medicine, UKMMC, Universiti Kebangsaan Malaysia, Jalan Yaacob Latif, Cheras, 56000, Kuala Lumpur, Malaysia
  2. Department of Basic Medical Sciences, Kuliyyah of Medicine, International Islamic University of Malaysia, Kuantan, Pahang, Malaysia
  3. Division of Pathology, School of Medicine, International Medical University, Bukit Jalil, Kuala Lumpur, Malaysia
  4. Department of Anatomy, Faculty of Medicine, UKMMC, Universiti Kebangsaan Malaysia, Cheras, Kuala Lumpur, Malaysia
  5. Department of Pathology, Faculty of Medicine, UKMMC, Universiti Kebangsaan Malaysia, Cheras, Kuala Lumpur, Malaysia
  6. Faculty of Traditional and Complementary Medicine, Cyberjaya University College of Medical Sciences, Cyberjaya, Selangor, Malaysia
  
• 刊物主题：Biomedicine general; Human Physiology; Animal Physiology;
• 出版者：Springer Netherlands
• ISSN：1877-8755

文摘

Oxidative stress plays an important role in cardiovascular diseases. The study investigated the effects of dietary palm tocotrienol-rich fraction on homocysteine metabolism in rats fed a high-methionine diet. Forty-two male Wistar rats were randomly assigned to six groups. Five groups were fed with high-methionine diet (1 %) for 10 weeks. Groups 2 to 5 were also given dietary folate (8 mg/kg) and three doses of palm tocotrienol-rich fraction (30, 60 and 150 mg/kg) from week 6 to week 10. The last group was only given basal rat chow. High-methionine diet increased plasma homocysteine after 10 weeks, which was prevented by the supplementations of folate and high-dose palm tocotrienol-rich fraction. Hepatic S-adenosyl methionine (SAM) content was unaffected in all groups but S-adenosyl homocysteine (SAH) content was reduced in the folate group. Folate supplementation increased the SAM/SAH ratio, while in the palm tocotrienol-rich fraction groups, the ratio was lower compared with the folate. Augmented activity of hepatic cystathionine β-synthase and lipid peroxidation content by high-methionine diet was inhibited by palm tocotrienol-rich fraction supplementations (moderate and high doses), but not by folate. The supplemented groups had lower hepatic lipid peroxidation than the high-methionine diet. In conclusion, palm tocotrienol-rich fraction reduced high-methionine-induced hyperhomocysteinaemia possibly by reducing hepatic oxidative stress in high-methionine-fed rats. It may also exert a direct inhibitory effect on hepatic cystathionine β-synthase. Keywords Tocotrienol Methionine Homocysteine S-Adenosyl methionine S-Adenosyl homocysteine Cystathionine β-synthase