Glycerol Dehydratases: Biochemical Structures, Catalytic Mechanisms, and Industrial Applications in 1,3-Propanediol Production by Naturally Occurring and Genetically Engineered Bacterial Strains

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• 作者：Jian-zhong Liu ; Wu Xu ; Andrei Chistoserdov…
• 刊名：Applied Biochemistry and Biotechnology
• 出版年：2016
• 出版时间：July 2016
• 年：2016
• 卷：179
• 期：6
• 页码：1073-1100
• 全文大小：1,135 KB
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Biotechnology
  Biochemistry
  
• 出版者：Humana Press Inc.
• ISSN：1559-0291
• 卷排序：179

文摘

To date, two types of glycerol dehydratases have been reported: coenzyme B₁₂-dependent and coenzyme B₁₂-independent glycerol dehydratases. The three-dimensional structure of the former is a dimer of αβγ heterotrimer, while that of the latter is a homodimer. Their mechanisms of reaction are typically enzymatic radical catalysis. Functional radical in both the glycerol dehydratases is the adenosyl radical. However, the adenosyl radical in the former originates from coenzyme B₁₂ by homolytic cleavage, and that in the latter from S-adenosyl-methionine. Until some years ago, Clostridium butyricum VPI 1718 was the only microorganism known to possess B₁₂-independent glycerol dehydratase, but since then, several other bacteria with this unique capability have been identified. This article focuses on the glycerol dehydratases and on 1,3-propanediol production from glycerol by naturally occurring and genetically engineered bacterial strains containing glycerol dehydratase.KeywordsGlycerol dehydratase (GDH)1,3-Propanediol (PD)Biochemical structureCatalytic mechanismGlycerolCrude glycerolBioprocessing