Chagasic patients are able to respond against a viral antigen from influenza virus

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• 作者：Paola Lasso (1)
  Diana Mesa (1)
  Natalia Bola?os (2)
  Adriana Cuéllar (3)
  Fanny Guzmán (4)
  Zulma Cucunuba (5)
  Fernando Rosas (6)
  Víctor Velasco (6)
  Maria C Thomas (7)
  Manuel Carlos López (7)
  John Mario González (2)
  Concepción Judith Puerta (1) (8)
  
• 关键词：CD8+ T cells ; Chagas disease ; Non ; T. cruzi microbial antigen ; Nonspecific immune ; suppression
• 刊名：BMC Infectious Diseases
• 出版年：2012
• 出版时间：December 2012
• 年：2012
• 卷：12
• 期：1
• 全文大小：303KB
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  44. The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-2334/12/198/prepub
  
• 作者单位：Paola Lasso (1)
  Diana Mesa (1)
  Natalia Bola?os (2)
  Adriana Cuéllar (3)
  Fanny Guzmán (4)
  Zulma Cucunuba (5)
  Fernando Rosas (6)
  Víctor Velasco (6)
  Maria C Thomas (7)
  Manuel Carlos López (7)
  John Mario González (2)
  Concepción Judith Puerta (1) (8)
  
  1. Laboratorio de Parasitología Molecular, Pontificia Universidad Javeriana, Carrera 7 No. 43 -82, Bogotá, Colombia
  2. Grupo de Ciencias Básicas Médicas, Facultad de Medicina, Universidad de los Andes, Carrera 1 No. 18A -10, Bogotá, Colombia
  3. Grupo de Inmunobiología y Biología Celular, Pontificia Universidad Javeriana, Carrera 7 No. 43 -82, Bogotá, Colombia
  4. Núcleo de Biotecnología Curauma, Pontificia Universidad Católica de Valparaíso, Avenida Universidad 330, Valparaíso, Chile
  5. Grupo de Parasitología, Instituto Nacional de Salud, Avenida Calle 26 No. 51 -20, Bogotá, Colombia
  6. Fundación Clínica Abood Shaio, Diag. 115A No. 70C -75, Bogotá, Colombia
  7. Instituto de Parasitología y Biomedicina López Neyra, Consejo Superior de Investigaciones Científicas (IPBLN-CSIC), Parque Tecnológico de Ciencias de la Salud, Avda. del Conocimiento, s/n.18100, Granada, Spain
  8. Departamento de Microbiología, Facultad de Ciencias, Laboratorio de Parasitología Molecular, Pontificia Universidad Javeriana, Bogotá, Colombia
  

文摘

Background Trypanosoma cruzi, the etiological agent of Chagas-disease, is an obligate intracellular parasite which induces a CD8+ T cell immune response with secretion of cytokines and release of cytotoxic granules. Although an immune-suppressive effect of T. cruzi on the acute phase of the disease has been described, little is known about the capacity of CD8+ T cell from chronic chagasic patients to respond to a non-T. cruzi microbial antigen. Methods In the present paper, the frequency, phenotype and the functional activity of the CD8+ T cells specific from Flu-MP*, an influenza virus epitope, were determined in 13 chagasic patients and 5 healthy donors. Results The results show that Flu-MP* peptide specific CD8+ T cells were found with similar frequencies in both groups. In addition, Flu-MP* specific CD8+ T cells were distributed in the early or intermediate/late differentiation stages without showing enrichment of a specific sub-population. The mentioned Flu-MP* specific CD8+ T cells from chagasic patients were predominately TEM (CCR7- CD62L-), producing IL-2, IFNγ, CD107a/b and perforin, and did not present significant differences when compared with those from healthy donors. Conclusions Our results support the hypothesis that there is no CD8+ T cell nonspecific immune-suppression during chronic Chagas disease infection. Nonetheless, other viral antigens must be studied in order to confirm our findings.