Bioassay-guided evaluation of Dioscorea villosa -an acute and subchronic toxicity, antinociceptive and anti-inflammatory approach
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- 作者：Claudio Moreira Lima (1) (3)
Adriana Karla Lima (1) (3)
Marcelia G Dória Melo (1)
Mairim Russo Serafini (1)
Dênisson Lima Oliveira (3)
Enrik Barbosa de Almeida (3)
Rosana Souza Siqueira Barreto (1)
Paulo Cesar de Lima Nogueira (4)
Valéria Regina de Souza Moraes (4)
édica Ramone Andrade Oliveira (4)
Ricardo Luiz Cavalcanti de Albuquerque Jr (2) (3)
Lucindo J Quintans-Júnior (1)
Adriano Antunes Souza Araújo (1) - 关键词：Dioscorea villosa ; Toxicity ; Antinociceptive effect ; Anti ; inflammatory effect
- 刊名：BMC Complementary and Alternative Medicine
- 出版年：2013
- 出版时间：December 2013
- 年：2013
- 卷：13
- 期：1
- 全文大小：438KB
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55. The pre-publication history for this paper can be accessed here:ss="a-plus-plus">http://www.biomedcentral.com/1472-6882/13/195/prepub - 作者单位：Claudio Moreira Lima (1) (3)
Adriana Karla Lima (1) (3)
Marcelia G Dória Melo (1)
Mairim Russo Serafini (1)
Dênisson Lima Oliveira (3)
Enrik Barbosa de Almeida (3)
Rosana Souza Siqueira Barreto (1)
Paulo Cesar de Lima Nogueira (4)
Valéria Regina de Souza Moraes (4)
édica Ramone Andrade Oliveira (4)
Ricardo Luiz Cavalcanti de Albuquerque Jr (2) (3)
Lucindo J Quintans-Júnior (1)
Adriano Antunes Souza Araújo (1)
1. Department of Physiology, Federal University of Sergipe-UFS, S?o Cristóv?o, SE, CEP 49000-100, Brazil
3. Tiradentes University, Aracaju, SE, CEP 49000-000, Brazil
4. Department of Chemistry, Federal University of Sergipe, S?o Cristóv?o, SE, 49100-000, Brazil
2. Laboratory of Morphology and Structural Biology Science and Technology Institute -ITP, Aracaju, SE, CEP 49000-000, Brazil
文摘
Background Dioscorea villosa (DV) has been used in Brazil as an alternative medicine to attenuate menopause symptoms, as well as for the treatment of joint pain and rheumatoid arthritis. In spite of the popular use of DV for the treatment of various disorders, there are limited scientific data regarding safety aspects of this herb. In this regard, we carried out to evaluated both antinociceptive and anti-inflammatory activities in experimental models and assess the toxic effects of the acute (single dose) and subchronic (30?days) oral administration of dry extract of Dioscorea villosa in rodents. Methods The LC analyses were performed to assess the presence of the diosgenin in samples of DV. The antinociceptive study of DV was performed using models of acetic acid-induced writhing and formalin-induced pain in mice. The anti-inflammatory study was accomplished by leukocyte migration to the peritoneal cavity. A dry extract of DV was tested at doses of 100, 200 and 400?mg/kg (per os or p.o.). The toxicological properties of the dry extract were evaluated by toxicity assays of acute (5?g/kg, single dose) and subchronic (1?g/kg/day, 30?days) treatment. Haematological, biochemical, and histopathological parameters were studied. The results are expressed as mean?±?S.D., and statistical analysis of the data were performed with the Student’s t-test or one-way analysis of variance (ANOVA) followed by Tukey’s test. In all cases differences were considered significant if p-lt;-.05. Results HPLC-DAD analysis of the extract from DV revealed the presence of diosgenin as the major compound. Doses of 200 and 400?mg?kg significantly reduced the amount of acetic acid-induced writhing in relation to the vehicle (p-lt;-.0001). In the first phase, using the formalin-induced neurogenic pain test, only the 400?mg/kg dose of DV showed significant inhibition of neurogenic pain (p-lt;-.001). In the second phase, 200 and 400?mg/kg of DV showed significant inhibition of inflammatory pain (p-lt;-.0001). Significant inhibition of leukocyte migration was observed with doses of 100 (p-lt;-.001), 200 (p-lt;-.01) and 400?mg/kg (p-lt;-.01). Haematological, biochemical and histopathological data obtained in both acute and subchronic toxicological assays revealed only unremarkable changes, which are unlikely to indicate DV toxicity with oral administration. Conclusion We found that DV possesses antinociceptive and anti-inflammatory properties in rodent models. In addition, no acute or subchronic toxicity was evident when the herbal extract was administered orally. These results supporting the folkloric usage of the plant to treat various inflammatory diseases.