Growth-dependent surface characteristics of Hansenula Polymorpha: implications for expanded bed adsorption chromatography

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• 作者：Nadia Naz ; Roy N. Dsouza ; Vikas Yelemane…
• 关键词：expanded bed adsorption ; chromatography ; extended DLVO theory ; surface energetics ; cell adhesion ; hydrophobic interaction ; ion exchange ; microbial growth
• 刊名：Biotechnology and Bioprocess Engineering
• 出版年：2015
• 出版时间：June 2015
• 年：2015
• 卷：20
• 期：3
• 页码：576-584
• 全文大小：321 KB
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• 作者单位：Nadia Naz (1)
  Roy N. Dsouza (1)
  Vikas Yelemane (1)
  Rami Reddy Vennapusa (2)
  Martin Kangwa (1)
  Marcelo Fern谩ndez-Lahore (1)
  
  1. Downstream Bioprocessing Laboratory, School of Engineering and Science, Jacobs University, Campus Ring 1, D-28759, Bremen, Germany
  2. Manufacturing Technologies Laboratory, Shantha Biotechnics (A Sanofi Company), Hyderabad, 501-401, India
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Biotechnology
  
• 出版者：The Korean Society for Biotechnology and Bioengineering
• ISSN：1976-3816

文摘

The cell surface characteristics of a methylotrophic wild-type strain of yeast, Hansenula polymorpha, was investigated at different growth stages (early log, late log, stationary and death) of the biomass under different conditions (low and high salt in intact and disrupted forms) using extended DLVO theory. Biomass was characterized by contact angle measurements as well as zeta potential determinations. These measurements were used to describe the hydrophobic, polar, and electrostatic behavior of the biomass in its growth stages. Consequently, interaction free energy vs. distance profiles of the biomass with anion-exchange and HIC adsorbents were conveniently generated. A strong interaction was calculated between cells and the adsorbents in the stationary and death phases of the biomass illustrated by the striking correlation between theoretical predictions and biomass deposition experiments. The physico-chemical properties of biomass in different growth phases have important implications for expanded bed adsorption chromatography, where unfavorable biomass-adsorbent interactions adversely affect process efficiency.