Recovery of native protein from potato root water by expanded bed adsorption with amberlite XAD7HP
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- 作者：Fan-Kui Zeng ; Hong Liu ; Peng-Jun Ma ; Gang Liu
- 关键词：potato root water ; potato protein ; protease inhibitors ; expanded bed adsorption ; Amberlite XAD7HP
- 刊名：Biotechnology and Bioprocess Engineering
- 出版年：2013
- 出版时间：September 2013
- 年：2013
- 卷：18
- 期：5
- 页码：981-988
- 全文大小：431KB
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Hong Liu (2)
Peng-Jun Ma (1) (3)
Gang Liu (1)
1. Research & Development Center for Eco-material and Eco-Chemistry, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou, 730-000, China
2. Spice and Beverage Research Institute, Chinese Academy of Tropical Agricultural Sciences, Wanning, 571-533, China
3. University of Chinese Academy of Sciences, Beijing, 100-049, China - ISSN：1976-3816
文摘
Potato root water (PRW) contains ?.5% protein. In this study, expanded bed adsorption (EBA) chromatography with Amberlite XAD7HP resin adsorbent was used to isolate native protein from crude PRW. The optimal pH and ionic strength for potato protein binding onto Amberlite XAD7HP were 5.0 and 20 mmol/L. The EBA-refined proteins were dried by vacuum freeze drying and spray drying at varying outlet temperatures. Results indicated that low temperature spray drying was the most cost effective method with respect to retaining protease inhibitor activities. The dried protein concentrates appeared bright yellow or dark reddish brown, with a total glycoalkaloid content of ?70 μg/g. The protease inhibitor activity was ?00 mg/g and 11 ?12 mg/g for trypsin inhibition and chymotrypsin inhibition, respectively. The results presented here suggest that EBA using Amberlite XAD7HP as the adsorbent is a feasible strategy for the direct adsorption of native protein from crude PRW.