Direct adventitious shoot bud formation on hypocotyls explants in Millettia pinnata (L.) Panigrahi- a biodiesel producing medicinal tree species

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• 作者：Durga Singh Nagar ; Suman Kumar Jha…
• 关键词：In vitro ; Hypocotyl ; Direct adventitious bud ; Silver nitrate ; Adenine sulphate ; Milletia pinnata
• 刊名：Physiology and Molecular Biology of Plants
• 出版年：2015
• 出版时间：April 2015
• 年：2015
• 卷：21
• 期：2
• 页码：287-292
• 全文大小：536 KB
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• 作者单位：Durga Singh Nagar (1)
  Suman Kumar Jha (1)
  Jigar Jani (1)
  
  1. Department of Forest Biology and Tree Improvement, College of Forestry, Navsari Agricultural University, Navsari, Gujarat, 396 450, India
  
• 刊物主题：Plant Sciences; Plant Physiology; Biophysics and Biological Physics; Cell Biology;
• 出版者：Springer India
• ISSN：0974-0430

文摘

A reproducible protocol developed for in vitro regeneration of Milletia pinnata using hypocotyl segments. Multiple shoots were induced from hypocotyl explants through direct adventitious shoot bud regeneration. The proximal end of hypocotyls was responsive for shoot bud induction. Silver nitrate and adenine sulphate had a positive effect on shoot bud induction and elongation. The maximum response and number of shoot bud produced in media supplemented with 8.88?μM BAP with 108.6?μM adenine sulphate and 11.84?μM silver nitrate. Elongated shoots were harvested and successful rooting of microshoots achieved on MS media supplemented with 9.84?μM IBA, with 81.1?% rooting. Remaining shoot buds sub-cultured for further multiplication and elongation. Each subculture produced eight to nine elongated microshoots up to four subcultures. The rooted microshoots were successfully hardened and transferred to field.