Adventitious Rooting and Browning are Differentially Controlled by Auxin in Rooting-Recalcitrant Elegia capensis (Burm. f.) Schelpe

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• 作者：Inge Verstraeten ; Danny Geelen
• 关键词：Adventitious root ; Auxin ; Light quality ; In vitro propagation ; Recalcitrance
• 刊名：Journal of Plant Growth Regulation
• 出版年：2015
• 出版时间：September 2015
• 年：2015
• 卷：34
• 期：3
• 页码：475-484
• 全文大小：1,708 KB
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• 作者单位：Inge Verstraeten (1) (2)
  Danny Geelen (1)
  
  1. Department of Plant Production, Faculty of Bioscience Engineering, Ghent University, Coupure links 653, 9000, Ghent, Belgium
  2. Department of Plant Systems Biology, VIB (Flanders Institute for Biotechnology), Technologiepark 927, 9052, Ghent, Belgium
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Plant Sciences
  Cell Biology
  Agriculture
  Forestry
  
• 出版者：Springer New York
• ISSN：1435-8107

文摘

Industrial propagation of horticultural species involves adventitious root (AR) induction, which is difficult to establish in rooting-recalcitrant species. One of these recalcitrant species is Elegia capensis (Burm. f.) Schelpe. This monocotyledonous species was used to investigate the correlation between tissue browning and AR formation. To evaluate the root-inducing potential of various auxin treatments, a test system using stem segments of in vitro grown Elegia shoots was developed. In addition to exogenous auxin treatments, the effect of more sucrose in the medium and the effect of different light quality were evaluated. The auxins IAA, IBA, NAA, and 2,4-D did not induce roots under standard light conditions, which confirmed the strong rooting-recalcitrance of E. capensis. However, in the dark, IBA successfully induced roots in 20-0 % of the segments, depending on the auxin concentration applied. Excised stem segments showed browning upon incubation with auxins. Browning was dependent on auxin type and concentration, yet it did not correlate with the capacity to form roots because AR formation was observed on brown stem segments. We therefore conclude that browning does not cause rooting-recalcitrance in E. capensis. Keywords Adventitious root Auxin Light quality In vitro propagation Recalcitrance