Genetic mapping of diagnostic markers for the Lg ₂ locus conferring ligules in Triticum aestivum L. and derived from Aegilops tauschii Coss.

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• 作者：Y. Amagai ; T. Kuboyama ; N. Watanabe
• 关键词：Ligulelessness ; Microsatellite mapping ; Mutation ; Sequence diversity
• 刊名：Euphytica
• 出版年：2017
• 出版时间：March 2017
• 年：2017
• 卷：213
• 期：3
• 全文大小：
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Plant Sciences; Plant Genetics & Genomics; Plant Pathology; Plant Physiology; Biotechnology;
• 出版者：Springer Netherlands
• ISSN：1573-5060
• 卷排序：213

文摘

It has been suggested that the non-allelic lg₂ and Lgt alleles, that confer liguleless phenotypes, are present in chromosome 2D. We hypothesized that the naturally occurring liguleless phenotype present in polyploid wheat was a typical example of loss of dominant duplicated alleles, a process that has been described as genetic diploidisation. In the present study, we obtained the base sequence of the candidate of Lg₂ gene through a “blastn” query with the upstream region of HvLG, the candidate gene sequence for the ligule locus in barley (Hordeum vulgare), and developed deletion markers of the Lg₂ locus from Ae. tauschii. We applied the deletion markers to locate the Lg₂ locus in hexaploid wheat. The F₂ progeny of ANK-33/SynW-5 segregated in a 3 liguled:1 liguleless ratio. The Lg₂ gene was completely linked to markers G3489_2DL12del and G3489_2DL11del and segregated in a 1:2:1 ratio. Liguleless Mutant, a liguleless mutant of Aegilops tauchii, was determined by the dominant Lgt gene. In Ae. tauschii, the F₂ progeny of KU2126/Liguleless Mutant, marker Atlg_2DL19del segregated in a 1:2:1 ratio independently of the 3 liguleless:1 liguled segregation. The results indicate that three deletion markers can be used to mark the Lg₂ locus in chromosome 2D of synthetic and hexaploid wheat.