Bone marrow mesenchymal stem cells promote osteosarcoma cell proliferation and invasion

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• 作者：Fu-Xiang Yu (1)
  Wei-Jian Hu (1)
  Bin He (1)
  Yi-Hu Zheng (1)
  Qi-Yu Zhang (1)
  Lin Chen (2)
  
  1. Department of General Surgery ; The First Affiliated Hospital of Wenzhou Medical University ; Nan Bai Xiang Street ; Ouhai District ; Wenzhou ; Zhejiang ; 325002 ; China
  2. Department of Pathology ; The First Affiliated Hospital of Wenzhou Medical University ; Nan Bai Xiang Street ; Ouhai District ; Wenzhou ; Zhejiang ; 325002 ; China
  
• 关键词：Bone marrow mesenchymal stem cells ; Osteosarcoma
• 刊名：World Journal of Surgical Oncology
• 出版年：2015
• 出版时间：December 2015
• 年：2015
• 卷：13
• 期：1
• 全文大小：972 KB
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• 刊物主题：Surgical Oncology;
• 出版者：BioMed Central
• ISSN：1477-7819

文摘

Background Bone marrow-derived stem cells (BMSCs) are locally adjacent to the tumor tissues and may interact with tumor cells directly. The purpose of this study was to explore the effects of BMSCs on the proliferation and invasion of osteosarcoma cells in vitro and the possible mechanism involved. Methods BMSCs were co-cultured with osteosarcoma cells, and CCK-8 assay was used to measure cell proliferation. The ELISA method was used to determine the concentration of stromal cell-derived factor-1 (SDF-1) in the supernatants. Reverse transcription polymerase chain reaction (RT-PCR) was performed to detect the expression of CXCR4 in osteosarcoma cells and BMSCs. Matrigel invasion assay was performed to measure tumor cell invasion. Results SDF-1 was detected in the supernatants of BMSCs, but not in osteosarcoma cells. Higher CXCR4 mRNA levels were detected in the osteosarcoma cell lines compared to BMSCs. In addition, conditioned medium from BMSCs can promote the proliferation and invasion of osteosarcoma cells, and AMD3100, an antagonist for CXCR4, can significantly downregulate these growth-promoting effects. Conclusions BMSCs can promote the proliferation and invasion of osteosarcoma cells, which may involve the SDF-1/CXCR4 axis.