Highly polymorphic nuclear microsatellite markers reveal detailed patterns of genetic variation in natural populations of Yezo spruce in Hokkaido

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• 作者：Masakazu G. Iwazumi (1)
  Mineaki Aizawa (2)
  Atsushi Watanabe (3)
  Susumu Goto (4)
  
  1. Kansai Regional Breeding Office ; Forest Tree Breeding Center ; Forestry and Forest Products Research Institute ; 1043 Uetsukinaka ; Shoo ; Katsuta ; Okayama ; 709-4335 ; Japan
  2. Department of Forest Science ; Faculty of Agriculture ; Utsunomiya University ; 350 Mine-machi ; Utsunomiya ; Tochigi ; 321-8505 ; Japan
  3. Department of Forest Environmental Science ; Faculty of Agriculture ; Kyushu University ; 6-10-1 Hakozaki ; Higashi-ku ; Fukuoka ; 812-8581 ; Japan
  4. Research Division ; The University Forests ; Graduate School of Agricultural and Life Sciences ; The University of Tokyo ; 1-1-1 Yayoi ; Bunkyo-ku ; Tokyo ; 113-8657 ; Japan
  
• 关键词：Afforestation ; Genetic structure ; Nuclear microsatellites ; Picea jezoensis ; Seed zone
• 刊名：Journal of Forest Research
• 出版年：2015
• 出版时间：April 2015
• 年：2015
• 卷：20
• 期：2
• 页码：301-307
• 全文大小：411 KB
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• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Forestry
  Forestry Management
  Tree Biology
  Plant Sciences
  Plant Ecology
  
• 出版者：Springer Japan
• ISSN：1610-7403

文摘

The Yezo spruce (Picea jezoensis) has attracted attention as a species commonly used for afforestation in Hokkaido. However, there is no information available for assessing appropriate seed and planting zones for this species. We developed 21 nuclear microsatellite markers for P. jezoensis and used them to examine the genetic structure of nine natural populations (288 trees). Based on the 31 samples, the 21 markers were highly polymorphic, with an average expected heterozygosity greater than 0.84. When we applied ten effective markers to nine populations in Hokkaido, no clear regional differences in genetic diversity were detected. STRUCTURE analysis revealed that, aside from certain geographically isolated or peripheral populations, the populations within planting zones belonged to a single cluster. Thus, based on our marker data, with a few exceptions, there appears to be a single seed zone within the planting zones in Hokkaido.