Identification of Ligament Intra-Crystalline Peptide (LICP) from the Hinge Ligament of the Bivalve, Pinctada Fucata

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• 作者：Michio Suzuki (1)
  Toshihiro Kogure (2)
  Shohei Sakuda (1)
  Hiromichi Nagasawa (1) (3)
  
  1. Department of Applied Biological Chemistry ; Graduate School of Agricultural and Life Sciences ; The University of Tokyo ; 1-1-1 Yayoi ; Bunkyo-ku ; Tokyo ; 113-8657 ; Japan
  2. Department of Earth and Planetary Science ; Graduate School of Science ; The University of Tokyo ; 7-3-1 Hongo ; Bunkyo-ku ; Tokyo ; 113-0033 ; Japan
  3. College of Life Science ; Zhejiang University ; Hangzhou ; Zhejiang ; 310058 ; People鈥檚 Republic of China
  
• 关键词：Aragonite ; Biomineralization ; Intra ; crystalline peptide ; Ligament ; Pinctada fucata
• 刊名：Marine Biotechnology
• 出版年：2015
• 出版时间：April 2015
• 年：2015
• 卷：17
• 期：2
• 页码：153-161
• 全文大小：1,204 KB
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• 刊物类别：Earth and Environmental Science
• 刊物主题：Earth sciences
  Oceanography
  
• 出版者：Springer New York
• ISSN：1436-2236

文摘

The hinge ligament of the bivalve is an important hard tissue that functions to open and close the shells. The ligament contains a fibrous structure consisting of aragonite crystals surrounded by dense organic matrices. Although many matrix proteins have been identified from various shell microstructures in previous works, ligament-specific matrix proteins have not yet been reported. In this study, in order to reveal the formation mechanism of the fibrous aragonite crystals in the ligament of Pinctada fucata, we identified a novel, small acidic peptide, named ligament intra-crystalline peptide (LICP), from the aragonite crystal of the ligament that had been pre-treated with sodium hypochlorite to remove the inter-crystalline organic matrices. LICP consists of 10 amino acid residues with N-terminal pyroglutamic acid. The result of cDNA cloning showed that the cDNA encodes another putative 10-residue peptide at the C-terminal end of LICP. LICP showed inhibitory activity on calcium carbonate precipitation, while the synthetic 10-residue peptide from the C-terminal sequence of proLICP did not. We also noted that the TEM and SEM observations of aragonite crystals formed by the in vitro crystallization experiment showed that LICP inhibited the growth of aragonite crystal to stop elongation in the c-axis direction. These results suggested that LICP has a role of regulating the formation of the aragonite crystals in the ligament.