The Effects of RegM on Stress Responses in Brucella melitensis

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• 作者：Hao Dong (2)
  Wenjuan Liu (3)
  Xiaowei Peng (1)
  Qingmin Wu (1)
  
  2. Department of Zoonosis Control ; China Animal Disease Control Center ; Beijing ; China
  3. Department of Radiation Oncology ; Shandong Cancer Hospital and Institute ; Jinan ; China
  1. Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture ; College of Veterinary Medicine ; China Agricultural University ; Beijing ; 100193 ; China
  
• 刊名：Current Microbiology
• 出版年：2015
• 出版时间：May 2015
• 年：2015
• 卷：70
• 期：5
• 页码：730-734
• 全文大小：520 KB
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• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Microbiology
  Biotechnology
  
• 出版者：Springer New York
• ISSN：1432-0991

文摘

Brucella melitensis is a facultative intracellular pathogen. The regM gene encodes a sensory transduction protein kinase in B. melitensis 16M, and genes orthologous to regM have been found to exist in many bacterial species. However, little is known about the regulation function of this gene in Brucella. In order to characterize this gene, we constructed a marked deletion mutant of regM as well as its complemented strain. The mutant was less able to withstand acid and hyperosmotic conditions than wild-type strain but shown no significant difference with wild-type strain when challenged by elevated temperature and hypotonic conditions. In addition, inactivation of regM did not affect virulence in B. melitensis 16M in macrophage and mice infection models.