Recombinant expressions of sweet plant protein mabinlin II in Escherichia coli and food-grade Lactococcus lactis

详细信息    查看全文

• 作者：Wenliang Gu (3)
  Qiyu Xia (1)
  Jing Yao (2)
  Shaoping Fu (1)
  Jianchun Guo (1)
  Xinwen Hu (2)
  
  3. Spice and Beverage Research Institute ; Chinese Academy of Tropical Agricultural Sciences ; Wanning ; China
  1. Key Laboratory of Biology and Genetic Resources of Tropical Crops ; Ministry of Agriculture ; Institute of Tropical Bioscience and Biotechnology ; Chinese Academy of Tropical Agricultural Sciences ; Haikou ; China
  2. College of Agriculture ; Hainan University ; Haikou ; China
  
• 关键词：Sweet protein ; Mabinlin II ; Recombinant expression ; Escherichia coli ; Lactococcus lactis
• 刊名：World Journal of Microbiology & Biotechnology
• 出版年：2015
• 出版时间：April 2015
• 年：2015
• 卷：31
• 期：4
• 页码：557-567
• 全文大小：4,615 KB
• 参考文献：1. Assadi-Porter, FM, Aceti, DJ, Cheng, H, Markley, JL (2000) Efficient production of recombinant brazzein, a small, heat-stable, sweet-tasting protein of plant origin. Arch Biochem Biophys 376: pp. 252-258 CrossRef
  2. Berlec, A, Jevnikar, Z, Majhenic, AC, Rogelj, I, Strukelj, B (2006) Expression of the sweet-tasting plant protein brazzein in Escherichia coli and Lactococcus lactis: a path toward sweet lactic acid bacteria. Appl Microbiol Biotechnol 73: pp. 158-163 CrossRef
  3. Berlec, A, Tompa, G, Slapar, N, Fonovic, UP, Rogelj, I, Strukelj, B (2008) Optimization of fermentation conditions for the expression of sweet-tasting protein brazzein in Lactococcus lactis. Lett Appl Microbiol 46: pp. 227-231 CrossRef
  4. Bogovic-Matijasic, B, Rogelj, I, Batic, M, Raspor, P (2001) Influence of pH on bacteriocin production by Lactobacillus K7 during batch fermentation. Period Biol 103: pp. 163-167
  5. Cheigh, CI, Choi, HJ, Park, H, Kim, SB, Kook, MC, Kim, TS, Hwang, JK, Pyun, YR (2002) Influence of growth conditions on the production of a nisin-like bacteriocin by Lactococcus lactis subsp. lactis A164 isolated from kimchi. J Biotechnol 95: pp. 225-235 CrossRef
  6. Chen, Z, Cai, H, Lu, F, Du, L (2005) High-level expression of a synthetic gene encoding a sweet protein, monellin, in Escherichia coli. Biotechnol Lett 27: pp. 1745-1749 CrossRef
  7. Chen, Z, Heng, C, Li, Z, Liang, X, Xinchen, S (2007) Expression and secretion of a single-chain sweet protein monellin in Bacillus subtilis by sacB promoter and signal peptide. Appl Microbiol Biotechnol 73: pp. 1377-1381 CrossRef
  8. Daniell, S, Mellits, KH, Faus, I, Connerton, I (2000) Refolding the sweet-tasting protein thaumatin II from insoluble inclusion bodies synthesised in Escherichia coli. Food Chem 71: pp. 105-110 CrossRef
  9. Ruyter, PG, Kuipers, OP, Vos, WM (1996) Controlled gene expression systems for Lactococcus lactis with the food-grade inducer nisin. Appl Environ Microbiol 62: pp. 3662-3667
  10. Ding, M, Hu, Z (1986) Study on mabinlin, a sweet protein from the seeds of C. masaikai Levl. IV: stability and denaturation. Acta Bot Yunanica 8: pp. 181-192
  11. Elliker, PR, Anderson, AW, Hannesson, G (1956) An agar medium for lactic acid streptococci and lactobacilli. J Dairy Sci 39: pp. 1611-1612 CrossRef
  12. Faus, I (2000) Recent developments in the characterization and biotechnological production of sweet-tasting proteins. Appl Microbiol Biotechnol 53: pp. 145-151 CrossRef
  13. Holo, H, Nes, IF Transformation of Lactococcus by electroporation. In: Nickoloff, JA eds. (1995) Methods in molecular biology. Humana Press, Totowa, pp. 195-199
  14. Hu, Z, He, M (1983) Study on mabinlin, a sweet protein from the seeds of C. masaikai Levl. I: extraction, purification and certain characteristics. Acta Bot Yunanica 5: pp. 207-212
  15. Hu, Z, Liang, HX, Liu, XZ (1985) Study on mabinlin, a sweet protein from the seeds of C. masaikai Levl. III: storage site and state. Acta Bot Yunanica 7: pp. 187-194
  16. Hu, Z, Peng, LP, He, M (1985) Study on mabinlin, a sweet protein from the seeds of C. masaikai Levl. II: comparison of mabinlin I to mabinlin II. Acta Bot Yunanica 7: pp. 1-10
  17. Kondo, K, Miura, Y, Sone, H, Kobayashi, K, Iijima, H (1997) High-level expression of a sweet protein, monellin, in the food yeast Candida utilis. Nat Biotechnol 15: pp. 453-457 CrossRef
  18. Li, C, Bai, JH, Cai, ZL, Fan, OY (2002) Optimization of a culture medium for bacteriocin production by Lactococcus lactis using response surface methodology. J Biotechnol 93: pp. 27-34 CrossRef
  19. Li, XD, Staszewski, L, Xu, H, Durick, K, Zoller, M, Adler, E (2002) Human receptors for sweet and umami taste. PNAS 99: pp. 4692-4696 CrossRef
  20. Li, DF, Jiang, PH, Zhu, DY, Hu, YL, Max, M, Wang, DC (2008) Crystal structure of mabinlin II: a novel structural type of sweet proteins and the main structural basis for its sweetness. J Struct Biol 162: pp. 50-62 CrossRef
  21. Liu, XZ, Maeda, S, Hu, Z, Aiuchi, T, Nakaya, K, Kurihara, Y (1993) Purification, complete amino acid sequence and structural characterization of the heat-stable sweet protein, mabinlin II. Eur J Biochem 211: pp. 281-287 CrossRef
  22. Masuda, T, Tamaki, S, Kaneko, R, Wada, R, Fujita, Y, Mehta, A, Kitabatake, N (2004) Cloning, expression, and characterization of recombinant sweet-protein thaumatin II using the methylotrophic yeast Pichia pastoris. Biotechnol Bioeng 85: pp. 761-769 CrossRef
  23. Mierau, I, Olieman, K, Mond, J, Smid, EJ (2005) Optimization of the Lactococcus lactis nisin-controlled gene expression system NICE for industrial applications. Microb Cell Fact 4: pp. 16 CrossRef
  24. Nirasawa, S, Nishino, T, Katahira, M, Uesugi, S, Hu, Z, Kurihara, Y (1994) Structures of heat-stable and unstable homologues of the sweet protein mabinlin. The difference in the heat stability is due to replacement of a single amino acid residue. Eur J Biochem 223: pp. 989-995 CrossRef
  25. Nirasawa, S, Masuda, Y, Nakaya, K, Kurihara, Y (1996) Cloning and sequencing of a cDNA encoding a heat-stable sweet protein, mabinlin II. Gene 181: pp. 225-227 CrossRef
  26. Sambrook, J, Russell, DW (2001) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, New York
  27. Sch盲gger, H (2006) Tricine鈥揝DS-PAGE. Nat Protoc 1: pp. 16-22 CrossRef
  28. Zhang, XJ, Duan, GC, Zhang, RG (2009) Optimized expression of Helicobacter pylori ureB gene in the Lactococcus lactis nisin-controlled gene expression (NICE) system and experimental study of its immunoreactivity. Curr Microbiol 58: pp. 308-314 CrossRef
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Applied Microbiology
  Biotechnology
  Biochemistry
  Environmental Biotechnology
  Microbiology
  
• 出版者：Springer Netherlands
• ISSN：1573-0972

文摘

Sweet plant proteins, which are safe, natural, low-calorie sweeteners, may be suitable replacements for sugars in the food and beverage industries. Mabinlin II, a sweet plant protein, shows the most pronounced heat stability and acid resistance of any of the six known types of plant sweet proteins. However, mabinlin II is difficult to extract from the Capparis masaikai plant, which is itself becoming increasingly scarce. This limits the use of naturally acquired mabinlin II. In this study, recombinant mabinlin II proteins were expressed and purified in Escherichia coli and in food-grade Lactococcus lactis. Recombinant mabinlin II proteins MBL-BH (containing the B-chains of mabinlin II downstream fused with His-tag) and MBL-ABH (containing the A- and B-chains of mabinlin II downstream fused with His-tag) were expressed in E. coli in the form of inclusion bodies. They were then purified and renatured. The refolded MBL-BH was found to be 100 times sweeter than sucrose by weight, but it was not heat-stable. Refolded MBL-ABH was neither sweet nor heat-stable. Recombinant mabinlin II proteins were secreted and expressed intracellularly in food-grade L. lactis, in which the concentrated cell samples and culture medium samples were detected using enzyme-linked immunosorbent assay and Western blotting analysis with anti-mabinlin II polyclonal antibody. This study demonstrated that the single B chain of mabinlin II has a sweet taste. The recombinant mabinlin II proteins have been successfully expressed in food-grade L. lactis, which is a crucial step in the production of mabinlin II through microorganism expression systems.