Systemic nematicidal activity and biocontrol efficacy of Bacillus firmus against the root-knot nematode Meloidogyne incognita

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• 作者：Jing Xiong (1)
  Qiaoni Zhou (1)
  Haiyan Luo (1)
  Liqiu Xia (1)
  Lin Li (2)
  Ming Sun (2)
  Ziquan Yu (1)
  
  1. State Key Laboratory of Breeding Base of Microbial Molecular Biology ; College of Life Science ; Hunan Normal University ; 36 Lushan Road ; Changsha ; 410081 ; People鈥檚 Republic of China
  2. State Key Laboratory of Agricultural Microbiology ; College of Life Science and Technology ; Huazhong Agricultural University ; Wuhan ; 430070 ; People鈥檚 Republic of China
  
• 关键词：Bacillus firmus ; Meloidogyne incognita ; Systemic nematicidal activity ; Biocontrol efficacy
• 刊名：World Journal of Microbiology & Biotechnology
• 出版年：2015
• 出版时间：April 2015
• 年：2015
• 卷：31
• 期：4
• 页码：661-667
• 全文大小：315 KB
• 参考文献：1. Chitwood, DJ (2003) Research on plant-parasitic nematode biology conducted by the United States Department of Agriculture鈥擜gricultural Research Service. Pest Manag Sci 59: pp. 748-753 CrossRef
  2. Ediz, SA, Dickerson, OJ (1976) Life cycle, pathogenicity, histopathology, and host range of race 5 of the barley root-knot nematode. J Nematol 8: pp. 228-232
  3. Giannakou, IO, Karpouzas, DG, Prophetou-Athanasiadou, D (2004) A novel non-chemical nematicide for the control of root-knot nematodes. Appl Soil Ecol 26: pp. 69-79 CrossRef
  4. Huang, G, Allen, R, Davis, EL, Baum, TJ, Hussey, RS (2006) Engineering broad root-knot resistance in transgenic plants by RNAi silencing of a conserved and essential root-knot nematode parasitism gene. Proc Natl Acad Sci USA 103: pp. 14302-14306 CrossRef
  5. Hussey, RS, Barker, RK (1973) A comparison of methods of collecting inocula of Meloidogyne spp. including a new technique. Plant Dis Rep 57: pp. 1025-1028
  6. Jaubert, S, Milac, AL, Petrescu, AJ, Almeida-Engler, J, Abad, P, Rosso, MN (2005) In planta secretion of a calreticulin by migratory and sedentary stages of root-knot nematode. Mol Plant Microbe Interact 18: pp. 1277-1284 CrossRef
  7. Jenkins, WR (1964) A rapid centrifugal-flotatioin technique for separating nematodes from soil. Plant Dis Rep 48: pp. 492
  8. Keren-Zur, M, Antonov, J, Bercovitz, A, Feldman, K, Husid, A, Kenan, G, Marcov, N, Rebhun, M (2000) Bacillus firmus formulations for the safe control of root-knot nematodes. Proc Brighton Crop Prot Conf Pests Dis 2A: pp. 47-52
  9. Kiewnick, S, Sikora, RA (2006) Evaluation of Paecilomyces lilacinus strain 251 for the biological control of the norther root-knot nematode Meloidogyne hapla chitwood. Nematology 8: pp. 69-78 CrossRef
  10. Lord, JS, Lazzeri, L, Atkinson, HJ, Urwin, PE (2011) Biofumigation for control of pale potato cyst nematodes: activity of Brassica leaf extracts and green manures on Globodera pallida in vitro and in soil. J Agric Food Chem 59: pp. 7882-7890 CrossRef
  11. Mendoza, AR, Sikora, RA (2009) Biological control of Radopholus similis in banana by combined application of the mutualistic endophyte Fusarium oxysporum strain162, the egg pathogen Paecilomyces lilacinus strain 251 and the antagonistic bacteria Bacillus firmus. Biocontrol 54: pp. 263-272 CrossRef
  12. Mendoza, AR, Kiewnick, S, Sikora, RA (2008) In vitro activity of Bacillus firmus against the burrowing nematode Radopholus similis, the root-knot nematode Meloidogyne incognita and the stem nematode Ditylenchus dipsaci. Biocontrol Sci Technol 18: pp. 377-389 CrossRef
  13. Meyer, SL (2003) United States Department of Agriculture鈥擜gricultural Research Service research programs on microbes for management of plant-parasitic nematodes. Pest Manag Sci 59: pp. 665-670 CrossRef
  14. Morton, CO, Hirsch, PR, Kerry, BR (2004) Infection of plant-parasitic nematodes by nematophagous fungi鈥攁 review of the application of molecular biology to understand infection processes and to improve biological control. Nematology 6: pp. 161-170 CrossRef
  15. Oka, Y, Shuker, S, Tkachi, N (2012) Systemic nematicidal activity of fluensulfone against the root-knot nematode Meloidogyne incognita on pepper. Pest Manag Sci 68: pp. 268-275 CrossRef
  16. Schneider, SM, Rosskopf, EN, Leesch, JG, Chellemi, DO, Bull, CT, Mazzola, M (2003) United States Department of Agriculture鈥擜gricultural Research Service research on alternatives to methyl bromide: pre-plant and post-harvest. Pest Manag Sci 59: pp. 814-826 CrossRef
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  19. Terefe, M, Tefera, T, Sakhuja, PK (2009) Effect of a formulation of Bacillus firmus on root-knot nematode Meloidogyne incognita infestation and the growth of tomato plants. J Invertebr Pathol 100: pp. 94-99 CrossRef
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• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Applied Microbiology
  Biotechnology
  Biochemistry
  Environmental Biotechnology
  Microbiology
  
• 出版者：Springer Netherlands
• ISSN：1573-0972

文摘

A strain of marine bacterium Bacillus firmus YBf-10 with nematicidal activity was originally isolated by our group. In the present study, the systemic nematicidal activity and biocontrol efficacy in pot experiment of B. firmus YBf-10 were investigated. Our results showed that YBf-10 exhibits systemic nematicidal activity against Meloidogyne incognita, including lethal activity, inhibition of egg hatch and motility. Pot experiment suggested that soil drenching with YBf-10 efficiently reduced damage of M. incognita to tomato plants, such as reduction of galls, egg masses on roots, and final nematode population in soil; and moreover, YBf-10 significantly promoted host plant growth. In addition, our results also indicated that the systemic nematicidal activity is likely attributed to the secondary metabolites produced by YBf-10. The obtained results of the current study confirmed that B. firmus YBf-10 is a promising nematicidal agent, and has great potential in plant-parasitic nematicidal management.