Infection of Beet necrotic yellow vein virus with RNA4-encoded P31 specifically up-regulates pathogenesis-related protein 10 in Nicotiana benthamiana

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• 作者：Wen-Qi Wu (5)
  Hui-Yan Fan (5)
  Ning Jiang (5)
  Ying Wang (5)
  Zong-Ying Zhang (5)
  Yong-Liang Zhang (5)
  Xian-Bing Wang (5)
  Da-Wei Li (5)
  Jia-Lin Yu (5)
  Cheng-Gui Han (5) (6)
  
  5. State Key Laboratory of Agrobiotechnology and the Ministry of Agriculture Key Laboratory for Plant Pathology ; China Agricultural University ; Beijing ; 100193 ; China
  6. Department of Plant Pathology ; China Agricultural University ; Beijing ; 100193 ; China
  
• 关键词：Beet necrotic yellow vein virus ; RNA4 ; P31 ; Pathogenesis ; related protein 10 ; Nicotiana benthamiana
• 刊名：Virology Journal
• 出版年：2014
• 出版时间：December 2014
• 年：2014
• 卷：11
• 期：1
• 全文大小：1,583 KB
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• 刊物主题：Virology;
• 出版者：BioMed Central
• ISSN：1743-422X

文摘

Background Beet necrotic yellow vein virus (BNYVV) is the infectious agent of sugar beet rhizomania, which consists of four or five plus-sense RNAs. RNA4 of BNYVV is not essential for virus propagation in Nicotiana benthamiana but has a major effect on symptom expression. Early reports showed that RNA4-encoded P31 was associated with severe symptoms, such as curling and dwarfing, in N. benthamiana. Results We discovered that the pathogenesis-related protein 10 (PR-10) gene can be up-regulated in BNYVV-infected N. benthamiana in the presence of RNA4 and that it had a close link with symptom development. Our frame-shift, deletion and substitution analysis showed that only the entire P31 could induce PR-10 up-regulation during BNYVV infection and that all the tryptophans and six cysteines (C174, C183, C186, C190, C197 and C199) in the cysteine-rich P31 had significant effects on PR-10 expression. However, P31 could not interact directly with PR-10 in yeast. Conclusions Our data demonstrated that only integrated P31 specifically induced PR-10 transcription, which coincided closely with the appearance of severe symptoms in BNYVV-infected N. benthamiana, although they could not interact directly with each other in yeast.