Nitrate reductase-mediated synthesis of silver nanoparticles from AgNO₃

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• 作者：S. Anil Kumar ; Majid Kazemian Abyaneh ; S. W. Gosavi ; Sulabha K. Kulkarni ; Renu Pasricha ; Absar Ahmad and M. I. Khan
• 关键词：Capping peptide ; Enzymatic method ; Nitrate reductase ; Silver nanoparticles
• 刊名：Biotechnology Letters
• 出版年：2007
• 出版时间：March, 2007
• 年：2007
• 卷：29
• 期：3
• 页码：439-445
• 全文大小：274 KB

文摘

Synthesis of silver nanoparticles using α-NADPH-dependent nitrate reductase and phytochelatin in vitro has been demonstrated for the first time. The silver ions were reduced in the presence of nitrate reductase, leading to the formation of a stable silver hydrosol 10–25 nm diam. and stabilized by the capping peptide. The nanoparticles were characterized by X-ray diffraction, transmission electron microscopy, X-ray photoelectron spectroscopy and UV-Vis absorption. These studies will help in designing a rational enzymatic strategy for the synthesis of nanomaterials of different chemical composition, shapes and sizes as well as their separation.