Expression of neurexin and neuroligin in the enteric nervous system and their down-regulated expression levels in Hirschsprung disease

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• 作者：Qiangye Zhang (1)
  Jian Wang (1)
  Aiwu Li (1)
  Hongzhen Liu (1)
  Wentong Zhang (1)
  Xinhai Cui (1)
  Kelai Wang (1)
  
• 关键词：Neurexin ; Neuroligin ; Hirschsprung disease ; Mesenteric plexus ; Neurons
• 刊名：Molecular Biology Reports
• 出版年：2013
• 出版时间：April 2013
• 年：2013
• 卷：40
• 期：4
• 页码：2969-2975
• 全文大小：547KB
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• 作者单位：Qiangye Zhang (1)
  Jian Wang (1)
  Aiwu Li (1)
  Hongzhen Liu (1)
  Wentong Zhang (1)
  Xinhai Cui (1)
  Kelai Wang (1)
  
  1. Department of Pediatric Surgery, Qilu Hospital, Shandong University, 107 Wenhuaxi Road, Jinan, 250012, Shandong, China
  
• ISSN：1573-4978

文摘

To investigate the expression levels of neurexins and neuroligins in the enteric nervous system (ENS) in Hirschsprung Disease (HSCR). Longitudinal muscles with adherent mesenteric plexus were obtained by dissection of the fresh gut wall of mice, guinea pigs, and humans. Double labeling of neurexin I and Hu (a neuron marker), neuroligin 1 and Hu, neurexin I and synaptophysin (a presynaptic marker), and neuroligin 1 and PSD95 (a postsynaptic marker) was performed by immunofluorescence staining. Images were merged to determine the relative localizations of the proteins. Expression levels of neurexin and neuroligin in different segments of the ENS in HSCR were investigated by immunohistochemistry. Neurexin and neuroligin were detected in the mesenteric plexus of mice, guinea pigs, and humans with HSCR. Neurexin was located in the presynapse, whereas neuroligin was located in the postsynapse. Expression levels of neurexin and neuroligin were significant in the ganglionic colonic segment of HSCR, moderate in the transitional segment, and negative in the aganglionic colonic segment. The expressions of neurexin and neuroligin in the transitional segments were significantly down-regulated compared with the levels in the normal segments (P?<?0.05). Expression levels of neurexin and neuroligin in ENS are significantly down-regulated in HSCR, which may be involved in the pathogenesis of HSCR.