GmPGIP3 enhanced resistance to both take-all and common root rot diseases in transgenic wheat

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• 作者：Aiyun Wang ; Xuening Wei ; Wei Rong ; Liang Dang…
• 关键词：Wheat ; Polygalacturonase ; inhibiting protein ; GmPGIP3 ; Gaeumannomyces graminis var. tritici ; Bipolaris sorokiniana
• 刊名：Functional & Integrative Genomics
• 出版年：2015
• 出版时间：May 2015
• 年：2015
• 卷：15
• 期：3
• 页码：375-381
• 全文大小：1,282 KB
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• 作者单位：Aiyun Wang (1) (2)
  Xuening Wei (2)
  Wei Rong (1) (2)
  Liang Dang (2) (3)
  Li-Pu Du (2)
  Lin Qi (2)
  Hui-Jun Xu (2)
  Yanjun Shao (3)
  Zengyan Zhang (2)
  
  1. College of Life Science and Technology, Central South University of Forestry and Technology, Changsha, 410004, China
  2. The National Key Facility for Crop Gene Resources and Genetic Improvement, Key Laboratory of Crop Genetic and Breeding of Ministry of Agriculture, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, 100081, China
  3. College of Biology Science, Agricultural University of Hebei, Baoding, 071001, China
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Cell Biology
  Plant Genetics and Genomics
  Microbial Genetics and Genomics
  Biochemistry
  Bioinformatics
  Animal Genetics and Genomics
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1438-7948

文摘

Take-all (caused by the fungal pathogen Gaeumannomyces graminis var. tritici, Ggt) and common root rot (caused by Bipolaris sorokiniana) are devastating root diseases of wheat (Triticum aestivum L.). Development of resistant wheat cultivars has been a challenge since no resistant wheat accession is available. GmPGIP3, one member of polygalacturonase-inhibiting protein (PGIP) family in soybean (Glycine max), exhibited inhibition activity against fungal endopolygalacturonases (PGs) in vitro. In this study, the GmPGIP3 transgenic wheat plants were generated and used to assess the effectiveness of GmPGIP3 in protecting wheat from the infection of Ggt and B. sorokiniana. Four independent transgenic lines were identified by genomic PCR, Southern blot, and reverse transcription PCR (RT-PCR). The introduced GmPGIP3 was integrated into the genomes of these transgenic lines and could be expressed. The expressing GmPGIP3 protein in these transgenic wheat lines could inhibit the PGs produced by Ggt and B. sorokiniana. The disease response assessments postinoculation showed that the GmPGIP3-expressing transgenic wheat lines displayed significantly enhanced resistance to both take-all and common root rot diseases caused by the infection of Ggt and B. sorokiniana. These data suggested that GmPGIP3 is an attractive gene resource in improving resistance to both take-all and common root rot diseases in wheat.