RP-HPLC-DAD Determination of Phenolics in Industrial Apple Pomace

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• 作者：Shalika Rana (1) (2)
  Ajay Rana (1) (3)
  Ashu Gulati (1) (3)
  Shashi Bhushan (1) (2)
  
• 关键词：Antioxidants ; Industrial apple pomace ; Phenolics ; RP ; HPLC ; Phloridzin
• 刊名：Food Analytical Methods
• 出版年：2014
• 出版时间：August 2014
• 年：2014
• 卷：7
• 期：7
• 页码：1424-1432
• 全文大小：883 KB
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• 作者单位：Shalika Rana (1) (2)
  Ajay Rana (1) (3)
  Ashu Gulati (1) (3)
  Shashi Bhushan (1) (2)
  
  1. Academy of Scientific and Innovative Research (AcSIR), CSIR-Institute of Himalayan Bioresource Technology, Palampur, India
  2. Food and Nutraceutical Lab, Division of Biotechnology, CSIR-Institute of Himalayan Bioresource Technology, Palampur, 176 061, Himachal Pradesh, India
  3. Hill Area Tea Science Division, CSIR-Institute of Himalayan Bioresource Technology, Palampur, 176 061, Himachal Pradesh, India
  
• ISSN：1936-976X

文摘

Processing of apple fruits for juice/cider production generates large volume of pomace at industrial scale. This biomass (cell wall material and pulp tissues) is rich in array of nutrients, especially dietary fiber and polyphenols. Apple fruits confront various processing conditions at industrial level, influencing the biochemical composition of generated pomace, including its phenolic profile. In present study, a simple, fast and reproducible reversed-phase high-performance liquid chromatography method using diode array detector was developed and validated for separation of different phenolics present in industrial apple pomace. The present method showed reliable and reproducible intraday (0.2-.0?% RSD) and interday (3.7-.1?% RSD) precision with limits of detection and quantification values in range of 0.14-.58 and 0.48-.95?μg?ml?, respectively, for all the phenolics. Different solvent-mediated extraction of dried pomace powder was also performed to evaluate its total antioxidant potential using standard spectrophotometric assays. The major phenolics found in industrial apple pomace extracts were quercetin (1.4-0.3?μg?mg?), phloretin (1.1-.3?μg?mg?) and phloridzin (0.62-.0?μg?mg?).