On the use of [18F]DOPA as an imaging biomarker for transplanted islet mass

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• 作者：Olof Eriksson (1)
  Akiva Mintz (2)
  Chengyang Liu (2)
  Ming Yu (2)
  Ali Naji (2)
  Abass Alavi (2)
  
• 关键词：Islet imaging ; FDOPA ; Positron emission tomography
• 刊名：Annals of Nuclear Medicine
• 出版年：2014
• 出版时间：January 2014
• 年：2014
• 卷：28
• 期：1
• 页码：47-52
• 全文大小：476 KB
• 作者单位：Olof Eriksson (1)
  Akiva Mintz (2)
  Chengyang Liu (2)
  Ming Yu (2)
  Ali Naji (2)
  Abass Alavi (2)
  
  1. Department of Medicinal Chemistry, Preclinical PET Platform, Uppsala University, Uppsala, Sweden
  2. Perelman School of Medicine at the University of Pennsylvania, 3400 Spruce Street, Philadelphia, PA, USA
  
• ISSN：1864-6433

文摘

Aim Islet transplantation is being developed as a potential cure for patients with type 1 diabetes. There is a need for non-invasive imaging techniques for the quantification of transplanted islets, as current transplantation sites are associated with a substantial loss of islet viability. The dopaminergic metabolic pathway is present in the islets; therefore, we propose Fluorine-18 labeled l-3,4-dihydroxyphenylalanine ([18F]DOPA) as a biomarker for transplanted islet mass. Methods The expression of enzymes involved in the dopaminergic metabolic pathway was investigated in both native and transplanted human islets. The specific uptake of [18F]DOPA in islets and immortalized beta cells was studied in vitro by selective blocking of dopa decarboxylase (DDC). Initial in vivo PET imaging of viable subcutaneous human islets was performed using [18F]DOPA. Results DDC and vesicular monoamine transporter 2 are co-localized with insulin in the native human pancreas, and the expression is retained after transplantation. Islet uptake of the [18F]DOPA could be modulated by inhibiting DDC, indicating that the uptake followed the normal dopaminergic metabolic pathway. In vivo imaging revealed [18F]DOPA uptake at the site of the functional islet graft. Conclusion Based on the in vitro and in vivo results presented in this study, we propose to further validate [18F]DOPA-PET as a sensitive imaging modality for imaging extrahepatically transplanted islets.