Escherichia coli-based production of recombinant ovine angiotensinogen and its characterization as a renin substrate

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• 作者：Shinji Yamashita ; Naoya Shibata ; Akiyoshi Boku-Ikeda ; Erika Abe…
• 关键词：Angiotensinogen ; Renin ; Angiotensin ; Hypertension ; Plasma renin concentration ; E. coli ; Recombinant protein production ; Auto ; induction
• 刊名：BMC Biotechnology
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：16
• 期：1
• 全文大小：1,370 KB
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Biotechnology
  Life Sciences
  Plant Breeding/Biotechnology
  Stem Cells
  Transgenics
  
• 出版者：BioMed Central
• ISSN：1472-6750

文摘

Background Angiotensinogen (ANG) is a macromolecular precursor of angiotensin, which regulates blood pressure and electrolyte balance. ANG is specifically cleaved by renin, an aspartic protease, to initiate the angiotensin-processing cascade. Ovine ANG (oANG) from sheep plasma has been shown to be a better substrate for human renin, and it has been used in clinical renin assays. To expand the availability of oANG, we aimed to produce milligram levels of recombinant oANG using an Escherichia coli expression system.