文摘
A novel actinobacterial strain, designated CFH S0499T, was isolated from a soil sample collected from Catba island in Halong Bay, Vietnam. The cells were observed to be Gram-stain positive, aerobic, non-motile, curved rods. The strain was found to grow optimally at 28 °C and pH 7.0. Growth was found to occur at 0- % NaCl. Chemotaxonomically, the peptidoglycan type was determined to be of the A3α type, with glutamic acid, glycine, alanine and lysine as the major cell wall amino acids. The whole cell sugars were found to contain mannose, galactose, glucose, ribose and rhamnose. The polar lipids were identified as diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, glycolipids and two unidentified phospholipids. The major fatty acids were identified as anteiso-C15:0, iso-C15:0, anteiso-C17:0 and iso-C16:0 and the predominant respiratory quinone as MK-9 (H2), with a minor amount of MK-10 (H4) and MK-8 (H2). The G+C content of the genomic DNA was determined to be 71.8 mol%. The 16S rRNA gene sequence analysis showed that strain CFH S0499T should be assigned to the genus Sinomonas and is closely related to members of the species Sinomonas atrocyanea DSM 20127T (98.3 %), Sinomonas soli CW 59T (98.28 %), Sinomonas flava CW 108T (98.26 %), Sinomonas mesophila MPLK 26T (97.5 %) and Sinomonas notoginsengisoli SYP-B 575T (95.8 %). DNA–DNA hybridizations showed low values (49.1-4.5 %) between strain CFH S0499T and its four closest neighbours. Based on phenotypic, chemotaxonomic and phylogenetic analysis, strain CFH S0499T is concluded to represent a novel species of the genus Sinomonas, for which the name Sinomonas halotolerans sp. nov. is proposed, with CFH S0499T as the type strain (=CCTCC AB2014300T = KCTC 39116T). Keywords Sinomonas halotolerans sp. nov. Family Micrococcaceae Halong Bay