Caspase signalling pathways in human spermatogenesis

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• 作者：Carolina Almeida (1)
  Sofia Correia (2) (3)
  Eduardo Rocha (4)
  ?ngela Alves (5)
  Luís Ferraz (6)
  Joquina Silva (7)
  Mário Sousa (5) (7)
  Alberto Barros (1) (7)
  
• 关键词：Apoptosis ; Caspases ; Testis ; Obstructive azoospermia ; Non ; obstructive azoospermia
• 刊名：Journal of Assisted Reproduction and Genetics
• 出版年：2013
• 出版时间：April 2013
• 年：2013
• 卷：30
• 期：4
• 页码：487-495
• 全文大小：688KB
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• 作者单位：Carolina Almeida (1)
  Sofia Correia (2) (3)
  Eduardo Rocha (4)
  ?ngela Alves (5)
  Luís Ferraz (6)
  Joquina Silva (7)
  Mário Sousa (5) (7)
  Alberto Barros (1) (7)
  
  1. Department of Genetics, Faculty of Medicine, University of Porto, Porto, 4200-319, Portugal
  2. Department of Hygiene and Epidemiology, Faculty of Medicine, University of Porto, Porto, 4200-319, Portugal
  3. Institute of Public Health, University of Porto, Porto, 4050-600, Portugal
  4. Department of Microscopy, Laboratory of Histology, Institute of Biomedical Sciences Abel Salazar (ICBAS), UMIB, University of Porto, Porto, 4050-313, Portugal
  5. Department of Microscopy, Laboratory of Cell Biology, ICBAS, UMIB, University of Porto, Porto, 4050-313, Portugal
  6. Department of Urology, Hospital Centre of Vila Nova de Gaia, Vila Nova de Gaia, 4434-502, Portugal
  7. Centre for Reproductive Genetics Alberto Barros, Porto, 4100-009, Portugal
  
• ISSN：1573-7330

文摘

Purpose Little is known about the apoptotic mechanisms involved in abnormal spermatogenesis. In order to describe the significance of apoptosis in azoospermia, testicular tissue from abnormal spermatogenesis was analysed. Methods Testicular treatment biopsies were obtained from 27 men. Five presented oligozoospermia, 9 obstructive azoospermia (4 congenital bilateral absence of the vas deferens; 5 secondary azoospermia) and 13 non-obstructive azoospermia (5 hypospermatogenis; 3 maturation arrest; 5 Sertoli-cell-only syndrome). Immunohistochemical staining was performed for active caspases-3, ? and ?. The presence of active caspases in Sertoli cells and germ cells was analyzed using stereological tools. Results Increased active caspase-3 was found in Sertoli-cell-only syndrome. No significant differences were found in maturation arrest. In hypospermatogenesis, primary spermatocytes were the germ cells with higher active caspases. Oligozoospermia and secondary obstruction showed significant differences among germ cells for the presence of all active caspases. In oligozoospermia, spermatogonia presented significant increased active caspase-9 in relation to active caspase-8. In primary obstruction and hypospermatogenesis, germ cells presented significant increased active caspases-3 and ?. Conclusions Results suggest that increased active caspase-3 might be involved in Sertoli-cell-only syndrome etiology. In cases of hypospermatogenesis, intrinsic lesions at the meiotic stage seem to be related to the pathology. In secondary obstruction apoptosis is suggested to be initiated due to extrinsic and intrinsic lesions, whereas in primary obstruction only the intrinsic apoptotic pathway seems to be present. Finally, in oligozoospermic patients spermatogonia death by mitochondrial damage additionally to meiosis malfunctioning, might be on the origin of the decreased sperm output.