Yeasts isolated from Algerian infants's feces revealed a burden of Candida albicans species, non-albicans Candida species and Saccharomyces cer

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• 作者：Hamza Ait Seddik ; Alexandre Ceugniez ; Farida Bendali…
• 关键词：Infant feces ; Candida species ; Antagonistic yeasts ; Saccharomyces cerevisiae ; Probiotic
• 刊名：Archives of Microbiology
• 出版年：2016
• 出版时间：January 2016
• 年：2016
• 卷：198
• 期：1
• 页码：71-81
• 全文大小：655 KB
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• 作者单位：Hamza Ait Seddik (1)
  Alexandre Ceugniez (2)
  Farida Bendali (1)
  Benoit Cudennec (2)
  Djamel Drider (2)
  
  1. Laboratoire de Microbiologie Appliquée, Faculté des Sciences de la Nature et de la Vie, Université Abderrahmane Mira - Bejaia, 06000, Bejaïa, Algeria
  2. Laboratoire Régional de Recherche en Agroalimentaire et Biotechnologies, Institut Charles Viollette (EA 7394). Bâtiment Polytech’Lille, Université de Lille 1 Sciences et Technologies, Avenue Paul Langevin, Cité Scientifique, 59655, Villeneuve d’Ascq Cedex, France
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Microbiology
  Microbial Ecology
  Biochemistry
  Cell Biology
  Biotechnology
  Ecology
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1432-072X

文摘

This study aimed at showing the yeast diversity in feces of Algerian infants, aged between 1 and 24 months, hospitalized at Bejaia hospital (northeast side of the country). Thus, 20 colonies with yeast characteristics were isolated and identified using biochemical (ID32C Api system) and molecular (sequencing of ITS1-5.8S-ITS2 region) methods. Almost all colonies isolated (19 strains) were identified as Candida spp., with predominance of Candida albicans species, and one strain was identified as Saccharomyces cerevisiae. Screening of strains with inhibitory activities unveiled the potential of Candida parapsilosis P48L1 and Candida albicans P51L1 to inhibit the growth of Escherichia coli ATCC 25922 and Staphylococcus aureus ATCC 25923. Further studies performed with these two Candida strains revealed their susceptibility to clinically used antifungal compounds and were then characterized for their cytotoxicity and hemolytic properties. On the other hand, Saccharomyces cerevisiae P9L1 isolated as well in this study was shown to be devoid of antagonism but resulted safe and overall usable as probiotic.