Antifungal Activity of Microgramma vacciniifolia Rhizome Lectin on Genetically Distinct Fusarium oxysporum f. sp. lycopersici Races

详细信息    查看全文

• 作者：Lidiane Pereira de Albuquerque (1)
  Giselly Maria de Sá Santana (1)
  Thiago Henrique Napole?o (1)
  Luana Cassandra Breitenbach Barroso Coelho (1)
  Márcia Vanusa da Silva (1)
  Patrícia Maria Guedes Paiva (1)
  
• 关键词：Microgramma vacciniifolia ; Rhizome lectin ; Antifungal activity ; Fusarium oxysporum f. sp. lycopersici ; ISSR
• 刊名：Applied Biochemistry and Biotechnology
• 出版年：2014
• 出版时间：January 2014
• 年：2014
• 卷：172
• 期：2
• 页码：1098-1105
• 全文大小：190 KB
• 作者单位：Lidiane Pereira de Albuquerque (1)
  Giselly Maria de Sá Santana (1)
  Thiago Henrique Napole?o (1)
  Luana Cassandra Breitenbach Barroso Coelho (1)
  Márcia Vanusa da Silva (1)
  Patrícia Maria Guedes Paiva (1)
  
  1. Departamento de Bioquímica, Centro de Ciências Biológicas, Universidade Federal de Pernambuco, Recife, Pernambuco, 50670-420, Brazil
  
• ISSN：1559-0291

文摘

Fusarium oxysporum f. sp. lycopersici races 1, 2, and 3 deteriorate tomato crops since they cause a vascular wilt. Lectins are carbohydrate-binding proteins with hemagglutinating and antifungal activities. This work reports that Microgramma vacciniifolia rhizome lectin (MvRL) inhibits F. oxysporum f. sp. lycopersici race 3 growth (61?%) more intensely than of races 1 (55?%) and 2 (45?%). The hemagglutinating activity of MvRL was inhibited by glycoprotein preparations from mycelia of races 1, 2, and 3, and these data indicate that lectin carbohydrate-binding sites recognized glycosylated molecules from races. Inter-simple sequence repeat (ISSR) marker system showed that race 3 is genetically distinct from races 1 and 2, and thus the highest sensitiveness of F. oxysporum f. sp. lycopersici race 3 to MvRL may be due to molecular characteristics of this race.