Overexpression of cutaneous mitochondrial superoxide dismutase in recent-onset type 2 diabetes

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• 作者：Dan Ziegler ; Alexander Strom ; Jutta Brüggemann ; Iris Ziegler ; Bernd Ringel…
• 关键词：Heart rate variability ; Oxidative stress ; Peripheral nerve function ; Superoxide dismutase ; Sympathovagal balance ; Type 2 diabetes
• 刊名：Diabetologia
• 出版年：2015
• 出版时间：July 2015
• 年：2015
• 卷：58
• 期：7
• 页码：1621-1625
• 全文大小：507 KB
• 参考文献：1.Nishikawa T, Edelstein D, Du XL et al (2000) Normalizing mitochondrial superoxide production blocks three pathways of hyperglycaemic damage. Nature 404:787-90PubMed View Article
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• 作者单位：Dan Ziegler (1) (2) (3)
  Alexander Strom (1)
  Jutta Brüggemann (1)
  Iris Ziegler (1)
  Bernd Ringel (1)
  Sonja Püttgen (1)
  Michael Roden (1) (2) (3)
  for the GDS Group
  
  1. Institute for Clinical Diabetology, German Diabetes Center, Leibniz Center for Diabetes Research at Heinrich Heine University, Auf’m Hennekamp 65, 40225, Düsseldorf, Germany
  2. Department of Endocrinology and Diabetology, Medical Faculty, Heinrich Heine University, Düsseldorf, Germany
  3. German Center for Diabetes Research (DZD e.V.), Partner Düsseldorf, Düsseldorf, Germany
  
• 刊物类别：Medicine
• 刊物主题：Medicine & Public Health
  Internal Medicine
  Metabolic Diseases
  Human Physiology
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1432-0428

文摘

Aims/hypothesis Oxidative stress and microvascular damage have been implicated in the pathogenesis of diabetic neuropathy, with manganese superoxide dismutase 2 (SOD2) responsible for superoxide detoxification in mitochondria. We hypothesised that patients with recently diagnosed type 2 diabetes would show an altered cutaneous expression of SOD2 and endothelial cell area. Methods In this cross-sectional study, we assessed skin biopsies using immunohistochemistry, peripheral nerve function and heart rate variability in 69 participants of the German Diabetes Study with recently diagnosed type 2 diabetes and 51 control individuals. Results Subepidermal SOD2 area in the distal leg was increased by ~60% in the diabetic group vs the controls (0.24?±-.02% vs 0.15?±-.02%; p--.0005) and was correlated with an increasing duration of diabetes (r--.271; p--.024) and with the low frequency/high frequency ratio (β--.381; p--.002) as an indicator of sympathovagal balance. The area of the subepidermal endothelial cells (measured by CD31 staining) did not differ between the groups. Conclusions/interpretation Cutaneous antioxidative defence is enhanced in relation to the duration of diabetes and is linked to a cardiac sympathovagal imbalance towards a sympathetic predominance in individuals with recently diagnosed type 2 diabetes without evidence of endothelial cell damage. Whether cutaneous SOD2 levels can predict the development of diabetic neuropathy remains to be determined in prospective studies.