In vivo quantitative imaging of photoassimilate transport dynamics and allocation in large plants using a commercial positron emission tomography (PET) scanner
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- 作者：Abhijit A. Karve ; David Alexoff ; Dohyun Kim ; Michael J. Schueller…
- 关键词：Carbon allocation ; Positron emission tomography (PET) ; Transport ; Imaging ; Carbon ; 11 (11C)
- 刊名：BMC Plant Biology
- 出版年：2015
- 出版时间：December 2015
- 年：2015
- 卷：15
- 期：1
- 全文大小：3,154 KB
- 刊物主题：Plant Sciences; Agriculture; Tree Biology;
- 出版者：BioMed Central
- ISSN：1471-2229
文摘
Background Although important aspects of whole-plant carbon allocation in crop plants (e.g., to grain) occur late in development when the plants are large, techniques to study carbon transport and allocation processes have not been adapted for large plants. Positron emission tomography (PET), developed for dynamic imaging in medicine, has been applied in plant studies to measure the transport and allocation patterns of carbohydrates, nutrients, and phytohormones labeled with positron-emitting radioisotopes. However, the cost of PET and its limitation to smaller plants has restricted its use in plant biology. Here we describe the adaptation and optimization of a commercial clinical PET scanner to measure transport dynamics and allocation patterns of 11C-photoassimilates in large crops.