Ascaridia nymphii n. sp. (Nematoda: Ascaridida) from the alimentary tract of a severely emaciated dead cockatiel Nymphicus hollandicus

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• 作者：Niichiro Abe ; Kayoko Matsuo ; Ikuko Makino
• 关键词：Ascaridia nymphii n. sp. ; Cockatiel ; Nymphicus hollandicus ; Nuclear 18S ribosomal RNA gene ; Mitochondrial NADH dehydrogenase subunit 2 gene ; Cryptosporidium avian genotype V
• 刊名：Parasitology Research
• 出版年：2015
• 出版时间：November 2015
• 年：2015
• 卷：114
• 期：11
• 页码：4281-4288
• 全文大小：1,097 KB
• 参考文献：Abe N, Makino I (2010) Multilocus genotypic analysis of Cryptosporidium isolates from cockatiels, Japan. Parasitol Res 106:1491鈥?497CrossRef PubMed
  Avcioglu H, Burgu A, B枚l眉kba艧 CS (2008) Ascaridia numidae (Leiper, 1908; Travassos, 1913) in rock partridge (Alectoris chukar) in Turkey. Parasitol Res 102:527鈥?30CrossRef PubMed
  Current WL, Upton SJ, Haynes TB (1986) The life cycle of Cryptosporidium baileyi n. sp. (Apicomplexa, Cryptosporidiidae) infecting chickens. J Protozool 33:289鈥?96CrossRef PubMed
  Hartwich G, Tscherner W (1979) Ascaridia platyceri n. sp. eine neue Spulwurmart aus Papageien. Angew Parasitol 20:63鈥?7PubMed
  Kajerova V, Barus V, Literak I (2004a) Nematodes from the genus Ascaridia parasitizing psittaciform birds: a review and determination key. Vet Med 49:217鈥?23
  Kajerova V, Barus V, Literak I (2004b) New records of Ascaridia platyceri (Nematoda) in parrots (Psittaciformes). Vet Med 49:237鈥?41
  Katakam KK, Nejsum P, Kyvsgaard NC, J酶rgensen CB, Thamsborg SM (2010) Molecular and parasitological tools for the study of Ascaridia population dynamics in chickens. Avian Pathol 39:81鈥?5CrossRef PubMed
  Kung CC (1949) Notes on some avian species of Ascaridia. J Helminthol 23:95鈥?06CrossRef PubMed
  Liu GH, Shao R, Li JY, Zhou DH, Li H, Zhu XQ (2013) The complete mitochondrial genomes of three parasitic nematodes of birds: a unique gene order and insights into nematode phylogeny. BMC Genomics 14:414PubMed Central CrossRef PubMed
  Mabon JL, Reid WM (1973) Tissue stages in the development of Ascaridia numidae from the guinea fowl (Numida meleagris). J Parasitol 59:1012鈥?015CrossRef
  Mines JJ (1979) Ascaridia sprenti, a new species of nematode in Australian parrots. Int J Parasitol 9:371鈥?79CrossRef
  Mines JJ, Green PE (1983) Experimental Ascaridia columbae infections in budgerigars. Aust Vet J 60:279鈥?80CrossRef PubMed
  Nakamura AA, Homem CG, da Silva AM, Meireles MV (2014) Diagnosis of gastric cryptosporidiosis in birds using a duplex real-time PCR assay. Vet Parasitol 205:7鈥?3CrossRef PubMed
  Qi M, Wang R, Ning C, Li X, Zhang L, Jian F, Sun Y, Xiao L (2011) Cryptosporidium spp. in pet birds: gastric diversity and potential public health significance. Exp Parasitol 128:336鈥?40CrossRef PubMed
  Ramadan HH, Znada NYA (1992) Morphology and life history of Ascaridia galli in the domestic fowl that are raised in Jeddah. J K A U Sci 4:87鈥?9
  Robbins KM, Ye W, Fletcher OJ (2011) Identification of Ascaridia numidae in guinea fowl (Numida meleagris) and association with elevated mortality. Avian Dis 55:151鈥?54CrossRef PubMed
  Ryan U, Hijjawi N (2015) New developments in Cryptosporidium research. Int J Parasitol 45:367鈥?73CrossRef PubMed
  Schock RC, Cooper R (1978) Internal parasitisms in captive birds. Mod Vet Pract 59:439鈥?43PubMed
  Webster WA (1982) Internal parasites found in exotic birds imported into Canada. Can Vet J 23:230PubMed Central PubMed
  Wehr EE, Hwong JC (1964) The life cycle and morphology of Ascaridia columbae (Gmelin, 1790) Travassos, 1913 (Nematoda: Ascarididae) in the domestic pigeon (Columba livia domestica). J Parasitol 50:131鈥?37CrossRef
  Yamaguti S (1961) Systema Helminthum. 3. The nematodes of vertebrates. Intersci. Publ, New York and London
  
• 作者单位：Niichiro Abe (1)
  Kayoko Matsuo (2)
  Ikuko Makino (3)
  
  1. Department of Microbiology, Osaka City Institute of Public Health and Environmental Sciences, Tojo-cho 8-34, Tennoji-ku, Osaka, 543-0026, Japan
  2. Gifu Prefectural Meat Inspection Office, Hayashi-machi 3-167-1, Ogaki, Gifu, 503-0015, Japan
  3. Fujisawa Avian Clinic, 2-1-13 Kugenuma-Ishigami, Fujisawa, Kanagawa, 251-0025, Japan
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Biomedicine
  Medical Microbiology
  Microbiology
  Immunology
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1432-1955

文摘

This report describes Ascaridia nymphii n. sp., a new species isolated from the alimentary tract of cockatiel Nymphicus hollandicus in Japan. More than 63 nematodes were found in the formalin-fixed small intestine, ventriculus, proventriculus and crop of a 48-day-old young cockatiel that died after exhibiting severe emaciation. No nematode eggs were observed in the faecal examination performed while the cockatiel was alive, but Cryptosporidium oocysts were found. The intestinal mucosa was damaged considerably. Male worms had two alate spicules, well-developed precloacal sucker and a tail with ventrolateral caudal alae and mainly 11 pairs of papillae. Nuclear partial (813 bp) 18S ribosomal RNA gene (18S rDNA) sequences obtained from two female samples were mutually identical. They respectively showed 99.1 and 98.6 % identities to those from Ascaridia numidae and Ascaridia galli. Phylogenetic analysis using this locus indicated the present nematode as Ascaridia species. The mitochondrial NADH dehydrogenase subunit 2 gene (nad2) sequences obtained for four samples were mutually identical. They respectively showed 98.7, 85.7 and 82.2 % identities with those from Ascaridia columbae, Ascaridia sp. and A. galli. Combining the morphological and sequencing data from two loci, the present nematode was identified as A. nymphii n. sp., which is closely related with A. columbae. This report is the first of a study examining the distribution of Ascaridia species in captive parrots in Japan. This study also identified the trachea and cloaca, like Cryptosporidium baileyi, as the possible location of Cryptosporidium avian genotype V in avian hosts. Keywords Ascaridia nymphii n. sp. Cockatiel Nymphicus hollandicus Nuclear 18S ribosomal RNA gene Mitochondrial NADH dehydrogenase subunit 2 gene Cryptosporidium avian genotype V