The invasive ergot Claviceps purpurea var. spartinae recently established in the European Wadden Sea on common cord grass is genetically homogeneous and the sclerotia contain high amounts of ergot
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- 作者：Christian Boestfleisch ; Astrid M. Drotleff…
- 关键词：Amplified fragment length polymorphism (AFLP) ; Claviceps purpurea var. spartinae ; Epimers ; Ergot alkaloids ; Internal transcribed spacer (ITS) ; Invasive species ; Spartina anglica ; Wadden Sea
- 刊名：European Journal of Plant Pathology
- 出版年：2015
- 出版时间：March 2015
- 年：2015
- 卷：141
- 期：3
- 页码：445-461
- 全文大小：691 KB
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17. Mulac, D., & Humpf, H. U. (2011). Cytotoxicity and accumulation of ergot alkaloids in human primary cells. / Toxicology, 282, 112-21.- 作者单位：Christian Boestfleisch (1)
Astrid M. Drotleff (2)
Waldemar Ternes (2)
Stefan Nehring (3)
Sylvie Pa?outová (4)
Jutta Papenbrock (1)
1. Institute of Botany, Leibniz University Hannover, Herrenh?user Stra?e 2, 30419, Hannover, Germany
2. Institute of Food Toxicology and Analytical Chemistry, Department of Analytical Chemistry, University of Veterinary Medicine Hannover, Foundation, Bischofsholer Damm 15, 30173, Hannover, Germany
3. Federal Agency for Nature Conservation, Konstantinstra?e 110, 53179, Bonn, Germany
4. Laboratory of the Genetics and Metabolism of Fungi, Institute of Microbiology CAS, Vídeňská 1083, 142 20, Prague 4, Czech Republic- 刊物类别：Biomedical and Life Sciences
- 刊物主题：Life Sciences
Plant Pathology
Plant Sciences
Ecology- 出版者：Springer Netherlands
- ISSN：1573-8469
- 作者单位：Christian Boestfleisch (1)
文摘
A well-established population of Claviceps purpurea var. spartinae R.A. Duncan & J.F. White was found on the invasive common cord-grass Spartina anglica C.E. Hubbard at two localities on the German North Sea coast. Additionally, 25 populations of S. anglica with an average air-line distance of 50?km between them along the coastline, starting from the Netherlands via the German coastline up to Denmark, were investigated. All S. anglica populations were heavily infected with C. purpurea var. spartinae (lineage G3). Genetic characterization of the C. purpurea DNA using Amplified Fragment Length Polymorphism (AFLP) and Internal Transcribed Spacer (ITS) analysis clearly indicated that all populations collected at the Wadden Sea were grouped in the same epidemic clade. Comparisons with reference strains indicated that the isolates along the Wadden Sea belonged to the clade also found in Ireland and Wales. The ergot alkaloid pattern is very similar in all genotypes. Ergocristine, α-ergocryptine and their epimers were identified in the samples in high amounts comparable to average values in sclerotia found on rye. The sclerotia of C. purpurea var. spartinae are able to float on the water for several months and could thereby be distributed over large distances by seawater. The sclerotia found in the drift lines still contain the equally high contents of ergot alkaloids as those collected directly from the plants. Current data form the basis for risk assessments and predictions about further spreading of C. purpurea var. spartinae on S. anglica.