Characterization of thirteen new microsatellite markers for allis shad (Alosa alosa) and twaite shad (Alosa fallax)

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• 作者：Quentin Rougemont (1) (2)
  Anne-Laure Besnard (1) (2)
  Jean-Luc Baglini猫re (1) (2)
  Sophie Launey (1) (2)
  
  1. INRA ; UMR 985 Ecologie et Sant茅 des Ecosyst猫mes ; 65 rue de Saint-Brieuc ; 35042 ; Rennes ; France
  2. Agrocampus Ouest ; UMR ESE ; 65 rue de Saint-Brieuc ; 35042 ; Rennes ; France
  
• 关键词：Alosa alosa ; Alosa fallax ; Microsatellites ; Genetic structure ; Conservation genetics
• 刊名：Conservation Genetics Resources
• 出版年：2015
• 出版时间：March 2015
• 年：2015
• 卷：7
• 期：1
• 页码：259-261
• 全文大小：154 KB
• 参考文献：1. Aprahamian MW, Baglini猫re JL, Sabati茅 MR, Alexandrino PJ, Aprahamian CD (2002) / Alosa alosa and / Alosa fallax spp, litterature review and bibliography. R & D Technical Report W1-014/TR. Environment Agency, Bristol, UK
  2. Beeck P, Klinger H, Jatteau P, Chanseau M (2008) Re-introduction of allis shad to the River Rhine System: Netherlands, Germany & France. In: Soorae PS (ed.) Global re-introduction perspectives: re-introduction case-studies from around the globe. IUCN/SSC Re-introduction Specialist Group, Abu Dhabi, UAE, p viii聽+聽284
  3. Faria R, Wallner B, Weiss S, Alexandrino P (2004) Isolation and characterization of eight dinucleotide microsatellite loci from two closely related clupeid species ( / Alosa alosa and / A. fallax). Mol Ecol Notes 4:586鈥?88 CrossRef
  4. Malausa T, Gilles A, Megl茅cz E et al (2011) High-throughput microsatellite isolation through 454 GS-FLX Titanium pyrosequencing of enriched DNA libraries. Mol Ecol Resour 11:638鈥?44 CrossRef
  5. Schuelke M (2000) An economic method for the fluorescent labeling of PCR fragments. Nat Biotechnol 18:233鈥?34 CrossRef
  
• 刊物主题：Conservation Biology/Ecology; Plant Sciences; Animal Anatomy / Morphology / Histology; Evolutionary Biology;
• 出版者：Springer Netherlands
• ISSN：1877-7260

文摘

Allis shad Alosa alosa and twaite shad Alosa fallax are two related anadromous European clupeid species which have undergone a sharp decline in the past decades. We describe the development of 13 microsatellite markers for both species, based on 454 pyrosequencing technology. The number of alleles per locus ranged from 2 to 11 in A. alosa and from 4 to 13 in A. fallax. Gene diversity ranged from 0.364 to 0.800 for A. alosa and 0.226 to 0.849 for A. fallax. These markers will help in conservation genetics studies such as assessing the extent of population decline, defining appropriate conservation units and monitoring reintroduction programs.