Amperometric biosensor for hydrogen peroxide based on the co-electrodeposition of horseradish peroxidase and methylene blue on an ITO electrode modified with an anodic aluminum oxide template

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• 作者：Hui Yao (1) yaohui7225@163.com
  Huan Liu (1)
  Mei-jiao Sun (1)
  Lei Gong (2)
• 关键词：Horseradish peroxidase &#8211 ; Anodic aluminum oxide &#8211 ; Methylene blue &#8211 ; Electropolymerization &#8211 ; Biosensor
• 刊名：Microchimica Acta
• 出版年：2012
• 出版时间：April 2012
• 年：2012
• 卷：177
• 期：1-2
• 页码：31-37
• 全文大小：626.4 KB
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• 作者单位：1. College of Applied Chemistry, Shenyang University of Chemical Technology, Shenyang, 110142 People鈥檚 Republic of China2. College of Science, North University of China, Taiyuan, 030051 People鈥檚 Republic of China
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Analytical Chemistry
  Inorganic Chemistry
  Physical Chemistry
  Characterization and Evaluation Materials
  Monitoring, Environmental Analysis and Environmental Ecotoxicology
  
• 出版者：Springer Wien
• ISSN：1436-5073

文摘

We report on a nano-array sensor for hydrogen peroxide (H₂O₂) that is based on a nanoporous anodic aluminum oxide template. This was used as a matrix for the co-immobilization of horseradish peroxidase (HRP) and methylene blue (MB) on the surface of an indium tin oxide electrode. The immobilized HRP retained its natural activity and MB is capable of efficiently shuttle electrons between HRP and the electrode. The new electrode was characterized by SEM and electrochemical methods. It exhibits fast response, long-term stability, high sensitivity and good selectivity to H₂O₂. Under optimized conditions, it linearly responds to H₂O₂ in the concentration range from 1.0 μM to 26 mM, with a detection limit of 0.21 μM (at S/N = 3).