Responses of Proteolytic Enzymes in Embryonic Axes of Germinating Bean Seeds under Copper Stress

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• 作者：Inès Karmous (1)
  Khadija Jaouani (1)
  Ezzedine El Ferjani (1)
  Abdelilah Chaoui (1)
  
• 关键词：Aminopeptidases ; Bean ; Carboxypeptidases ; Copper ; Embryonic axes ; Endopeptidases
• 刊名：Biological Trace Element Research
• 出版年：2014
• 出版时间：July 2014
• 年：2014
• 卷：160
• 期：1
• 页码：108-115
• 全文大小：306 KB
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• 作者单位：Inès Karmous (1)
  Khadija Jaouani (1)
  Ezzedine El Ferjani (1)
  Abdelilah Chaoui (1)
  
  1. Plant Toxicology and Molecular Biology of Microorganisms, Faculty of Sciences of Bizerta, 7021, Zarzouna, Tunisia
  
• ISSN：1559-0720

文摘

The changes in protease activities in embryonic axes during the first days of bean (Phaseolus vulgaris L.) seed germination were investigated in response to copper stress. Synthetic substrates and specific protease inhibitors have been used to define qualitatively and quantitatively different catalytic classes, particularly endoproteases (EP), carboxypeptidases (CP) and aminopeptidases (AP), then identify which ones were affected in the presence of copper. In fact, a failure in storage proteins mobilization and a disorder of nitrogen supply at enzymatic level occurred in Cu. In fact, Cu inhibited azocaseinolytic activity (ACA) and cysteine-, aspartic-, serine-, and metallo-endopeptidases activities (Cys-EP, Asp-EP, Ser-Ep, and Met-EP, respectively). Besides, Cu affected leucine- and proline-aminopeptidases (LAP and PAP, respectively) and glycine-carboxypeptidases (Gly-CP). The proteolytic responses might also be associated with the decrease in defense capacity in the Cu-treated embryos.