PET Imaging of Cardiac Wound Healing Using a Novel [68Ga]-Labeled NGR Probe in Rat Myocardial Infarction
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- 作者：Jochen Tillmanns ; Magdalena Schneider ; Daniela Fraccarollo…
- 关键词：NGR ; CD13 ; RGD ; Myocardial infarction ; Wound healing ; Molecular imaging
- 刊名：Molecular Imaging and Biology
- 出版年：2015
- 出版时间：February 2015
- 年：2015
- 卷：17
- 期：1
- 页码：76-86
- 全文大小：11,666 KB
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文摘
Purpose Peptides containing the asparagine-glycine-arginine (NGR) motif bind to aminopeptidase N (CD13), which is expressed on inflammatory cells, endothelial cells, and fibroblasts. It is unclear whether radiolabeled NGR-containing tracers could be used for in vivo imaging of the early wound-healing phase after myocardial infarction (MI) using positron emission tomography (PET). Procedures Uptake of novel tracer [68Ga]NGR was assessed together with [68Ga]arginine-glycine-aspartic acid ([68Ga]RGD) and 2-deoxy-2-[18?F]fluoro-d-glucose after myocardial ischemia/reperfusion (MI/R) injury using μ-PET and autoradiography, and relative expressions of CD13 and integrin β3 were assessed in fibroblasts, inflammatory cells, and endothelial cells by immunohistochemistry. Results In the infarcted myocardium, uptake of [68Ga]NGR was maximal from days 3 to 7 after MI/R, and correlated with fibroblast and inflammatory cell infiltration as well as [68Ga]RGD uptake. Conclusions [68Ga]NGR allows noninvasive and sequential determination of CD13 expression in fibroblasts and inflammatory cells by PET. This will facilitate monitoring of CD13 in the individual wound healing processes, allowing patient-specific therapies to improve outcome after MI.