Glial cell line-derived neurotrophic factor induced the differentiation of amniotic fluid-derived stem cells into vascular endothelial-like cells in vitro

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• 作者：Ruyu Zhang ; Ying Lu ; Ju Li ; Jia Wang ; Caixia Liu…
• 关键词：Amniotic fluid ; derived stem cells ; Glial cell line ; derived neurotrophic factor ; Transfection ; Endothelial ; like cells
• 刊名：Journal of Molecular Histology
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：47
• 期：1
• 页码：9-19
• 全文大小：1,840 KB
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• 作者单位：Ruyu Zhang (1) (2)
  Ying Lu (1) (2)
  Ju Li (3)
  Jia Wang (1)
  Caixia Liu (1) (2)
  Fang Gao (4)
  Dong Sun (1) (2)
  
  1. Department of Nephrology, Affiliated Hospital of Xuzhou Medical College, 99 West Huai-hai Road, Xuzhou, 221002, Jiangsu, China
  2. Department of Internal Medicine and Diagnostics, Xuzhou Medical College, Xuzhou, 221002, China
  3. Huai’an First People’s Hospital, Nanjing Medical University, Huai’an, 223300, Jiangsu, China
  4. Department of Anesthesiology, Affiliated Hospital of Xuzhou Medical College, Xuzhou, 221002, China
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Cell Biology
  Biomedicine
  Developmental Biology
  
• 出版者：Springer Netherlands
• ISSN：1567-2387

文摘

Amniotic fluid-derived stem cells (AFSCs) are a novel source of stem cells that are isolated and cultured from second trimester amniocentesis. Glial cell line-derived neurotrophic factor (GDNF) acts as a tissue morphogen and regulates stem cell proliferation and differentiation. This study investigated the effect of an adenovirus-mediated GDNF gene, which was engineered into AFSCs, on the cells’ biological properties and whether GDNF in combination with AFSCs can be directionally differentiated into vascular endothelial-like cells in vitro. AFSCs were isolated and cultured using the plastic adherence method in vitro and identified by the transcription factor Oct-4, which is the primary marker of pluripotent stem cells. AFSCs were efficiently transfected by a GFP-labeled plasmid system of an adenovirus vector carrying the GDNF gene (Ad-GDNF-GFP). Transfected AFSCs stably expressed GDNF. Transfected AFSCs were cultured in endothelial growth medium-2 containing vascular endothelial growth factor. After 1 week, AFSCs were positive for von Willebrand factor (vWF) and CD31, which are markers of endothelial cells, and the recombinant GDNF group was significantly higher than undifferentiated controls and the GFP only group. These results demonstrated that AFSCs differentiated into vascular endothelial-like cells in vitro, and recombinant GDNF promoted differentiation. The differentiation-induced AFSCs may be used as seed cells to provide a new manner of cell and gene therapies for transplantation into the vascular injury site to promote angiogenesis. Keywords Amniotic fluid-derived stem cells Glial cell line-derived neurotrophic factor Transfection Endothelial-like cells