Characterization of a New Bacteriocin from Lactobacillus plantarum LE5 and LE27 Isolated from Ensiled Corn
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- 作者：Jairo Amortegui (1)
Alexander Rodríguez-López (1)
Deicy Rodríguez (2)
Ana K. Carrascal (2)
Carlos J. Alméciga-Díaz (1) (5)
Adelina del P. Melendez (3)
Oscar F. Sánchez (3) (4) - 关键词：Bacteriocins ; Lactobacillus plantarum ; Listeria monocytogenes antimicrobial activity
- 刊名：Applied Biochemistry and Biotechnology
- 出版年：2014
- 出版时间：April 2014
- 年：2014
- 卷：172
- 期：7
- 页码：3374-3389
- 全文大小：
- 参考文献：1. Rajilic-Stojanovic, M., Smidt, H., & de Vos, W. M. (2007). Diversity of the human gastrointestinal tract microbiota revisited. / Environmental Microbiology, 9, 2125-136. CrossRef
2. Wallace, T. C., Guarner, F., Madsen, K., Cabana, M. D., Gibson, G., Hentges, E., et al. (2011). Human gut microbiota and its relationship to health and disease. / Nutrition Reviews, 69, 392-03. CrossRef
3. Dicks, L. M. T., & Botes, M. (2010). Probiotic lactic acid bacteria in the gastro-intestinal tract: health benefits, safety and mode of action. / Benef Microbes, 1, 11-9. CrossRef
4. Masood, M. I., Qadir, M. I., Shirazi, J. H., & Khan, I. U. (2011). Beneficial effects of lactic acid bacteria on human beings. / Critical Reviews in Microbiology, 37, 91-8. CrossRef
5. Badel, S., Bernardi, T., & Michaud, P. (2011). New perspectives for lactobacilli exopolysaccharides. / Biotechnology Advances, 29, 54-6. CrossRef
6. Servin, A. L. (2004). Antagonistic activities of lactobacilli and bifidobacteria against microbial pathogens. / FEMS Microbiology Reviews, 28, 405-40. CrossRef
7. Benmechernene, Z., Fernandez-No, I., Kihal, M., B?hme, K., Calo-Mata, P., & Barros-Velazquez, J. (2013). Recent patents on bacteriocins: food and biomedical applications. / Recent Patents on DNA & Gene Sequences, 7, 66-3. CrossRef
8. Hammami, R., Fernandez, B., Lacroix, C., Fliss, I. (2012). Anti-infective properties of bacteriocins: an update. Cell. Mol. Life Sci.
9. Cotter, P. D., Ross, R. P., & Hill, C. (2013). Bacteriocins—a viable alternative to antibiotics? / Nature Reviews Microbiology, 11, 95-05. CrossRef
10. Cotter, P. D., Hill, C., & Ross, R. P. (2005). Bacteriocins: developing innate immunity for food. / Nature Reviews Microbiology, 3, 777-88. CrossRef
11. Drider, D., Fimland, G., Héchard, Y., McMullen, L. M., & Prévost, H. (2006). The continuing story of class IIa bacteriocins. / Microbiology and Molecular Biology Reviews, 70, 564-82. CrossRef
12. Papagianni, M., & Papamichael, E. M. (2011). Purification, amino acid sequence and characterization of the class IIa bacteriocin weissellin A, produced by / Weissella paramesenteroides DX. / Bioresource Technology, 102, 6730-734. CrossRef
13. Todorov, S. D., & Dicks, L. M. (2009). Bacteriocin production by / Pediococcus pentosaceus isolated from marula ( / Scerocarya birrea). / International Journal of Food Microbiology, 132, 117-26. CrossRef
14. Maldonado-Barragan, A., Caballero-Guerrero, B., Jimenez, E., Jimenez-Diaz, R., Ruiz-Barba, J. L., & Rodriguez, J. M. (2009). Enterocin C, a class IIb bacteriocin produced by / E. faecalis C901, a strain isolated from human colostrum. / International Journal of Food Microbiology, 133, 105-12. CrossRef
15. Todorov, S. D., Rachman, C., Fourrier, A., Dicks, L. M. T., van Reenen, C. A., Prévost, H., et al. (2011). Characterization of a bacteriocin produced by / Lactobacillus sakei R1333 isolated from smoked salmon. / Anaerobe, 17, 23-1. CrossRef
16. Hata, T., Tanaka, R., & Ohmomo, S. (2010). Isolation and characterization of plantaricin ASM1: a new bacteriocin produced by / Lactobacillus plantarum A-1. / International Journal of Food Microbiology, 137, 94-9. CrossRef
17. Mu?oz, M., Mosquera, A., Alméciga-Díaz, C. J., Melendez, A. P., & Sánchez, O. F. (2012). Fructooligosaccharides metabolism and effect on bacteriocin production in / Lactobacillus strains isolated from ensiled corn and molasses. / Anaerobe, 18, 321-30. CrossRef
18. Todorov, S. D., Ho, P., Vaz-Velho, M., & Dicks, L. M. (2010). Characterization of bacteriocins produced by two strains of / Lactobacillus plantarum isolated from Beloura and Chouri?o, traditional pork products from Portugal. / Meat Science, 84, 334-43. CrossRef
19. Sch?gger, H., & von Jagow, G. (1987). Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100?kDa. / Analytical Biochemistry, 166, 368-79. CrossRef
20. Campos, C. A., Rodríguez, ó., Calo-Mata, P., Prado, M., & Barros-Velázquez, J. (2006). Preliminary characterization of bacteriocins from / Lactococcus lactis, / Enterococcus faecium and / Enterococcus mundtii strains isolated from turbot ( / Psetta maxima). / Food Research International, 39, 356-64. CrossRef
21. Chevallet, M., Luche, S., & Rabilloud, T. (2006). Silver staining of proteins in polyacrylamide gels. / Nature Protocols, 1, 1852-858. CrossRef
22. Xu, D., & Zhang, Y. (2012). Ab initio protein structure assembly using continuous structure fragments and optimized knowledge-based force field. / Proteins, 80, 1715-735. CrossRef
23. Laskowski, R. A. (2009). PDBsum new things. / Nucleic Acids Research, 37, D355–D359. CrossRef
24. Martinez, R. C., Wachsman, M., Torres, N. I., LeBlanc, J. G., Todorov, S. D., & Franco, B. D. (2013). Biochemical, antimicrobial and molecular characterization of a noncytotoxic bacteriocin produced by / Lactobacillus plantarum ST71KS. / Food Microbiology, 34, 376-81. CrossRef
25. Nowroozi, J., Mirzaii, M., & Norouzi, M. (2004). Study of / Lactobacillus as probiotic bacteria. / Iranian Journal of Public Health, 33, 1-.
26. Cintas, L., Herranz, C., Hernández, P., Casaus, M., & Nes, L. (2001). Review: bacteriocins of lactic acid bacteria. / Food Science and Technology International, 7, 281-05. CrossRef
27. Todorov, S. D., & Dicks, L. M. (2005). Characterization of bacteriocins produced by lactic acid bacteria isolated from spoiled black olives. / Journal of Basic Microbiology, 45, 312-22. CrossRef
28. Todorov, S. D., & Dicks, L. M. T. (2006). Screening for bacteriocin-producing lactic acid bacteria from boza, a traditional cereal beverage from Bulgaria: comparison of the bacteriocins. / Process Biochemistry, 46, 11-9. CrossRef
29. Hammami, R., Zouhir, A., Le Lay, C., Ben Hamida, J., & Fliss, I. (2010). BACTIBASE second release: a database and tool platform for bacteriocin characterization. / BMC Microbiology, 10, 22. CrossRef
30. Todorov, S. D., van Reenen, C. A., & Dicks, L. M. (2004). Optimization of bacteriocin production by / Lactobacillus plantarum ST13BR, a strain isolated from barley beer. / Journal of General and Applied Microbiology, 50, 149-57. CrossRef
31. Zouhir, A., Hammami, R., Fliss, I., & Hamida, J. B. (2010). A new structure-based classification of gram-positive bacteriocins. / Protein Journal, 29, 432-39. CrossRef
32. Nes, I. F., & Johnsborg, O. (2004). Exploration of antimicrobial potential in LAB by genomics. / Current Opinion in Biotechnology, 15, 100-04. CrossRef
33. Gálvez, A., Abriouel, H., López, R. L., & Ben Omar, N. (2007). Bacteriocin-based strategies for food biopreservation. / International Journal of Food Microbiology, 120, 51-0. CrossRef
34. Todorov, S. D., & Dicks, L. M. T. (2005). / Lactobacillus plantarum isolated from molasses produces bacteriocins active against Gram-negative bacteria. / Enzyme and Microbial Technology, 36, 318-26. CrossRef
35. Zapata, S., Mu?oz, J., Ruiz, O., Montoya, O., & Gutierrez, P. (2009). Isolation of / Lactobacillus plantarum LPBM10 and partial characterization of its bacteriocin. / Vitae, 16, 75-2.
36. Mourad, K., Halima, Z.-K., & Nour-Eddine, K. (2005). Detection and activity of plantaricin OL15 a bacteriocin produced by / Lactobacillus plantarum OL15 isolated from Algerian fermented olives. / Grasas y Aceites, 56, 192-97. CrossRef
37. Sowani, H. M., & Thorat, P. (2012). Antimicrobial activity studies of bactoriocin produced by / Lactobacilli isolates from carrot kanji. / OnLine Journal of Biological Sciences, 12, 6-0. CrossRef
38. Lee, N. K., & Paik, H. D. (2001). Partial characterisation of lactocin NK24, a newly identified bacteriocin of / Lactococcus lactis NK24 isolated from Jeot-gal. / Food Microbiology, 18, 17-4. CrossRef
39. Ferchichi, M., Frère, J., Mabrouk, K., & Manai, M. (2001). Lactococcin MMFII, a novel class IIa bacteriocin produced by / Lactococcus lactis MMFII, isolated from a Tunisian dairy product. / FEMS Microbiology Letters, 205, 49-5. CrossRef
40. Todorov, S., & Dicks, L. M. T. (2005). Pediocin ST18, an anti-listerial bacteriocin produced by / Pediococcus pentosaceus ST18 isolated from boza, a traditional cereal beverage from Bulgaria. / Process Biochemistry, 40, 365-70. CrossRef
41. Huot, E., Meghrous, J., Barrena-Gonzalez, C., & Petitdemange, H. (1996). Bacteriocin J46, a new bacteriocin produced by / Lactococcus lactis subsp. / cremoris J46: isolation and characterization of the protein and its gene. / Anaerobe, 2, 137-45. CrossRef
42. Nissen-Meyer, J., Rogne, P., Oppeg?rd, C., Haugen, H. S., & Kristiansen, P. E. (2009). Structure-function relationships of the non-lanthionine-containing peptide (class II) bacteriocins produced by gram-positive bacteria. / Current Opinion in Biotechnology, 10, 19-7.
43. Gómez, N. C., Abriouel, H., Grande, M. A., Pulido, R. P., & Gálvez, A. (2012). Effect of enterocin AS-48 in combination with biocides on planktonic and sessile / Listeria monocytogenes. Food Microbiology, 30, 51-8. CrossRef
44. Brandt, A. L., Castillo, A., Harris, K. B., Keeton, J. T., Hardin, M. D., & Taylor, T. M. (2010). Inhibition of / Listeria monocytogenes by food antimicrobials applied singly and in combination. / Journal of Food Science, 75, M557–M563. CrossRef
45. Neetoo, H., Ye, M., & Chen, H. (2008). Potential antimicrobials to control / Listeria monocytogenes in vacuum-packaged cold-smoked salmon paté and fillets. / International Journal of Food Microbiology, 123, 220-27. CrossRef
46. Shelburne, C. E., An, F. Y., Dholpe, V., Ramamoorthy, A., Lopatin, D. E., & Lantz, M. S. (2007). The spectrum of antimicrobial activity of the bacteriocin subtilosin A. / Journal of Antimicrobial Chemotherapy, 59, 297-00. CrossRef
47. Mills, S., Serrano, L. M., Griffin, C., O'Connor, P. M., Schaad, G., Bruining, C., et al. (2011). Inhibitory activity of / Lactobacillus plantarum LMG P-26358 against / Listeria innocua when used as an adjunct starter in the manufacture of cheese. / Microbial Cell Factories, 10(Suppl 1), S7. CrossRef
48. Aguilar, C., Vanegas, C., Klotz, B. (2010). Antagonistic effect of / Lactobacillus strains against / Escherichia coli and / Listeria monocytogenes in milk. / The Journal of Dairy Research, 3, 1-.
49. Gong, H. S., Meng, X. C., & Wang, H. (2010). Mode of action of plantaricin MG, a bacteriocin active against / Salmonella typhimurium. Journal of Basic Microbiology, 50(Suppl 1), S37–S45. CrossRef - 作者单位：Jairo Amortegui (1)
Alexander Rodríguez-López (1)
Deicy Rodríguez (2)
Ana K. Carrascal (2)
Carlos J. Alméciga-Díaz (1) (5)
Adelina del P. Melendez (3)
Oscar F. Sánchez (3) (4)
1. Institute for the Study of Inborn Errors of Metabolism, School of Sciences, Pontificia Universidad Javeriana, Bogotá, Colombia
2. Laboratorio de Microbiología de alimentos, Grupo de Biotecnología Ambiental e Industrial, Departamento de Microbiología, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá, Colombia
5. Protein Expression and Purification Laboratory, Institute for the Study of Inborn Errors of Metabolism School of Science, Pontificia Universidad Javeriana, Cra 7 No. 43 E 82, Building 54, Room 303A, Bogotá, Colombia
3. Pharmacy Department, Universidad Nacional de Colombia, Carrera 30 No. 45-03, Building 450, Bogotá, Colombia
4. School of Chemical Engineering, Purdue University, West Lafayette, IN, USA - ISSN：1559-0291
文摘
Bacteriocins are low molecular peptides with antimicrobial activity, which are of great interest as food bio-preservatives and for treating diseases caused by pathogenic bacteria. In this study, we present the characterization of bacteriocins produced by Lactobacillus plantarum LE5 and LE27 isolated from ensiled corn. Bacteriocins were purified through ammonium sulfate precipitation and double dialysis by using 12- and 1-kDa membranes. Bacteriocins showed activity against Listeria innocua, Listeria monocytogenes, and Enteroccocus faecalis. Molecular weight was estimated through Tricine-SDS-PAGE and overloading the gel onto Mueller-Hinton agar seeded with L. monocytogenes, showing an inhibition zone between 5 and 10?kDa. NanoLC-MS/MS analysis allowed the identification of UPF0291 protein (UniProtKB/Swiss-Prot Q88VI7), which is also presented in other lactic acid bacteria without assigned function. Ab initio modeling showed it has an α-helix-rich structure and a large positive-charged region. Bacteriocins were stable between 4 and 121?°C and pH 2 and 12, and the activity was inhibited by SDS and proteases. Mode of action assay suggests that the bacteriocin causes of target microorganism. Taken together, these results describe a possible new class IIa bacteriocin produced by L. plantarum, which has a wide stability to physicochemical conditions, and that could be used as an alternative for the control of foodborne diseases.